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CA1026 Anti-Wilm's Tumor Protein Rabbit pAb

This Anti-Wilm's Tumor Protein Rabbit pAb is validated for use in Immunoblotting, Paraffin Sections, Immunoprecipitation for the detection of Wilm's Tumor Protein.

Anti-Wilm's Tumor Protein Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-WT-1

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CA1026
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, RRbPolyclonal Antibody
      Description
      OverviewRecognizes the ~47-55 kDa Wilm's tumor protein in Wilm's tumor tissue.
      Catalogue NumberCA1026
      Brand Family Calbiochem®
      SynonymsAnti-WT-1
      Application Data
      Detection of Wilm's tumor by immunohistochemistry. Sample: Wilm's tumor. Primary antibody: Anti-Wilm's Tumor Protein Rabbit pAb (Cat. No. CA1026) (1:100). Detection: fluorescence.
      References
      ReferencesHuff, V. 1998. Am. J. Med. Genet. 79, 260.
      Product Information
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.6.
      Positive controlWilm's tumor tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Paraffin Sections
      Application NotesImmunoblotting (see comments)
      Paraffin Sections (1:100, heat pre-treatment required)
      Immunoprecipitation (see comments)
      Application CommentsStaining of formalin-fixed, paraffin-embedded tissues requires antigen retrieval by boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min, followed by cooling at room temp for 20 min. This antibody is also reported to work for immunoblotting and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids from the C-terminus of human Wilm's tumor protein
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CA1026 0

      Documentation

      Anti-Wilm's Tumor Protein Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Wilm's Tumor Protein Rabbit pAb Certificates of Analysis

      TitleLot Number
      CA1026

      References

      Reference overview
      Huff, V. 1998. Am. J. Med. Genet. 79, 260.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-August-2007 RFH
      SynonymsAnti-WT-1
      ApplicationImmunoblotting (see comments)
      Paraffin Sections (1:100, heat pre-treatment required)
      Immunoprecipitation (see comments)
      Application Data
      Detection of Wilm's tumor by immunohistochemistry. Sample: Wilm's tumor. Primary antibody: Anti-Wilm's Tumor Protein Rabbit pAb (Cat. No. CA1026) (1:100). Detection: fluorescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~47-55 kDa Wilm's tumor protein.
      BackgroundWilm's tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm's tumor and mesothelioma.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids from the C-terminus of human Wilm's tumor protein
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlWilm's tumor tissue
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.6.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsStaining of formalin-fixed, paraffin-embedded tissues requires antigen retrieval by boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min, followed by cooling at room temp for 20 min. This antibody is also reported to work for immunoblotting and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage.
      Toxicity Standard Handling
      ReferencesHuff, V. 1998. Am. J. Med. Genet. 79, 260.