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07-355-S Anti-acetyl-Histone H3 (Lys23) Antibody, Trial Size

Use Anti-acetyl-Histone H3 (Lys23) Antibody (Rabbit Polyclonal Antibody), Trial Size / Pack validated in ChIP, WB to detect acetyl-Histone H3 (Lys23) also known as H3K23Ac, Histone H3 (acetyl K23).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST3H3, H3FT, MGC126886, H3t, MGC126888, H3T, H3/g, H3.4, H3/t UniProt Number: Q16695
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07-355-S
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Vrt, Yeast (S. cerevisiae)WB, DB, ChIPRbSerumPolyclonal Antibody
      Description
      Catalogue Number07-355-S
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-acetyl-Histone H3 (Lys23) Antibody, Trial Size
      Alternate Names
      • H3K23Ac
      • Histone H3 (acetyl K23)
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure.

      The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      References
      Product Information
      FormatSerum
      Control
      • Acid extracts from sodium butyrate treated HeLa cells
      PresentationRabbit antiserum containing 0.05% sodium azide and 30% glycerol
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-acetyl-Histone H3 (Lys23) Antibody (Rabbit Polyclonal Antibody), Trial Size / Pack validated in ChIP, WB to detect acetyl-Histone H3 (Lys23) also known as H3K23Ac, Histone H3 (acetyl K23).
      Key Applications
      • Western Blotting
      • Dot Blot
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation:
      Representative lot data.
      Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µL of either normal rabbit serum or 2 µL Anti-acetyl-Histone H3 (Lys23)and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of acetyl-Histone H3 (Lys23) associated DNA fragments were verified by qPCR using Control Primers specific for the human GAPDH promoter region as a positive locus, and MyoD primers as a negative locus. Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
      Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
      Western Blot Analysis:
      Representative lot data.
      Acid extracts from sodium butyrate treated HeLa cells (Lane 1, Catalog # 17-305) and recombinant Histone H3 (Lane 2, Catalog # 14-494) were probed with Anti-acetyl-Histone H3 (Lys23) (1:100,000 dilution).
      Arrow indicates acetyl histone H3 (~17 kDa)
      Dot Blot:
      Representative lot data.
      40 ng and 4ng amounts of histone peptides with various modifications (see table 1) were transferred to PVDF membrane and probed with Anti-Acetyl-Histone H3 (Lys23) antibody (1:2000 dilution). Proteins were visualized using a goat anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system. Image from a 60 second exposure is shown.
      Biological Information
      ImmunogenOvalbumin-conjugated, synthetic peptide (KQLASAcKAARK-C) corresponding to amino acids 18-27 of yeast histone H3 acetylated on lysine 23 with a C-terminal cysteine added for conjugation purposes
      HostRabbit
      SpecificityHistone H3 acetylated on lysine 23. Does not recognize unacetylated recombinant histone H3
      Species Reactivity
      • Human
      • Vertebrates
      • Yeast (S. cerevisiae)
      Species Reactivity NoteYeast, Human, Most common vertebrates
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • HIST3H3
      • H3FT
      • MGC126886
      • H3t
      • MGC126888
      • H3T
      • H3/g
      • H3.4
      • H3/t
      Modifications
      • Acetylation
      Purification MethodAntiserum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight17kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on acid extracts from sodium butyrate treated HeLa cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size10 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-355-S 04053252907494

      Documentation

      Anti-acetyl-Histone H3 (Lys23) Antibody, Trial Size SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-acetyl-Histone H3 (Lys23) Antibody, Trial Size Certificates of Analysis

      TitleLot Number
      Anti-acetyl-Histone H3 (Lys23), ChIP Grade, Trial Size - 3225741 3225741
      Anti-acetyl-Histone H3 (Lys23), ChIP Grade, Trial Size - 3386439 3386439
      Anti-acetyl-Histone H3 (Lys23), ChIP Grade, Trial Size - 3593750 3593750
      Anti-acetyl-Histone H3 (Lys23), ChIP Grade, Trial Size - 3729994 3729994
      Anti-acetyl-Histone H3 (Lys23), ChIP Grade, Trial Size - 3940950 3940950
      Anti-acetyl-Histone H3 (Lys23), ChIP Grade, Trial Size - 3974562 3974562
      Anti-acetyl-Histone H3 (Lys23), ChIP Grade, Trial Size - Q2187995 Q2187995
      Anti-acetyl-Histone H3 (Lys23), ChIP Grade, Trial Size -2625385 2625385
      Anti-acetyl-Histone H3 (Lys23), ChIP Grade, Trial Size Polyclonal Antibody 2900711