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OP25 Anti-c-N-ras (Ab-1) Mouse mAb (F155-227)

Anti-c-N-ras (Ab-1) Mouse mAb (F155-227) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
OP25
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, RMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~21 kDa c-N-Ras protein in neuronal cells. Does not react with c-H-Ras or c-K-Ras under conditions tested.
      Catalogue NumberOP25
      Brand Family Calbiochem®
      Application Data
      Detection of human c-N-Ras by immunoblotting: Sample: Whole cell lysate (56 µg) from MCF-7 cells. Primary antibody: Anti-c-N-Ras (Ab-1) Mouse mAb (F155-227) (Cat. No. OP25) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesRodenhuis, S., et al. 1992. Cancer Res. (Suppl) 52, 2665s.
      Sidransky, D., et al. 1992. Science 256, 102.
      Bos, J.L. 1989. Cancer Res. 49, 4682.
      Furth, M. E., et al. 1987. Oncogene 1, 47.
      Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
      Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
      Taparowsky, E., et al. 1983. Cell 34, 581.
      Ellis, R.W., et al. 1981. Nature 292, 506.
      Shih, T.Y., et al. 1980. Nature 287, 686.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Positive controlNeuronal cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Paraffin Sections
      Application NotesFrozen Sections (10 µg/ml)
      Immunoblotting (1-10 µg/ml)
      Immunoprecipitation (see comments)
      Paraffin Sections (10 µg/ml, saponin or pepsin pre-treatment required)
      Application CommentsThe level of expression of p21ras is variable in different tissues. For this reason a concentration step such as immunoprecepitation prior to immunoblot analysis is recommended to obtain optimal results. c-N-ras (Ab-1) reacts with c-N-ras and v-N-ras but not with c-H-ras or c-K-ras, under the conditions tested. For immunoprecipitation, use 5 µg antibody per reaction with 45 µl of Protein G plus-agarose (Cat. No. IP04). Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant p21 Ras
      CloneF155-227
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      OP25 0

      Documentation

      Anti-c-N-ras (Ab-1) Mouse mAb (F155-227) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-c-N-ras (Ab-1) Mouse mAb (F155-227) Certificates of Analysis

      TitleLot Number
      OP25

      References

      Reference overview
      Rodenhuis, S., et al. 1992. Cancer Res. (Suppl) 52, 2665s.
      Sidransky, D., et al. 1992. Science 256, 102.
      Bos, J.L. 1989. Cancer Res. 49, 4682.
      Furth, M. E., et al. 1987. Oncogene 1, 47.
      Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
      Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
      Taparowsky, E., et al. 1983. Cell 34, 581.
      Ellis, R.W., et al. 1981. Nature 292, 506.
      Shih, T.Y., et al. 1980. Nature 287, 686.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-October-2007 RFH
      ApplicationFrozen Sections (10 µg/ml)
      Immunoblotting (1-10 µg/ml)
      Immunoprecipitation (see comments)
      Paraffin Sections (10 µg/ml, saponin or pepsin pre-treatment required)
      Application Data
      Detection of human c-N-Ras by immunoblotting: Sample: Whole cell lysate (56 µg) from MCF-7 cells. Primary antibody: Anti-c-N-Ras (Ab-1) Mouse mAb (F155-227) (Cat. No. OP25) (1 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 myeloma cells. Recognizes the ~21 kDa c-N-ras protein.
      BackgroundThe human ras gene family consists of three identified members, H, K and N-ras, encoding proteins of 188-189 amino acids and 21,000 (p21) daltons. Human H- and K-ras are the homologues of v-H- and v-K-ras sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes can be activated to oncogenes by mutations occurring in codons 12, 13, and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of ras proto-oncogene proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21s and their relevance to human disease diagnosis and prognosis is still emerging.
      HostMouse
      Immunogenrecombinant p21 Ras
      CloneF155-227
      IsotypeIgG₁
      Specieshuman, mouse, rat
      Positive controlNeuronal cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThe level of expression of p21ras is variable in different tissues. For this reason a concentration step such as immunoprecepitation prior to immunoblot analysis is recommended to obtain optimal results. c-N-ras (Ab-1) reacts with c-N-ras and v-N-ras but not with c-H-ras or c-K-ras, under the conditions tested. For immunoprecipitation, use 5 µg antibody per reaction with 45 µl of Protein G plus-agarose (Cat. No. IP04). Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesRodenhuis, S., et al. 1992. Cancer Res. (Suppl) 52, 2665s.
      Sidransky, D., et al. 1992. Science 256, 102.
      Bos, J.L. 1989. Cancer Res. 49, 4682.
      Furth, M. E., et al. 1987. Oncogene 1, 47.
      Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
      Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
      Taparowsky, E., et al. 1983. Cell 34, 581.
      Ellis, R.W., et al. 1981. Nature 292, 506.
      Shih, T.Y., et al. 1980. Nature 287, 686.