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MABS2193-25UG Anti-gp91phox (NOX2) Antibody, clone NL7

Anti-gp91phox (NOX2), clone NL7, Cat. No. MABS2193, is a mouse monoclonal antibody that detects Cytochrome b-245 heavy chain (gp91phox) and is tested for use in Flow Cytometry, Inhibition Analysis, and Western Blotting.

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MABS2193-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS2193-25UG
      DescriptionAnti-gp91phox (NOX2) Antibody, clone NL7
      Alternate Names
      • Cytochrome b-245 heavy chain
      • CGD91-phox
      • Cytochrome b(558) subunit beta
      • Cytochrome b558 subunit beta
      • Heme-binding membrane glycoprotein gp91phox
      • NADPH oxidase 2
      • Neutrophil cytochrome b 91 kDa polypeptide
      • Superoxide-generating NADPH oxidase heavy chain subunit
      • gp91-1
      • p22 phagocyte B-cytochrome
      Background InformationCytochrome b-245 heavy chain (UniProt: P04839; also known as CGD91-phox, Cytochrome b(558) subunit beta, Cytochrome b558 subunit beta, Heme-binding membrane glycoprotein gp91phox, NADPH oxidase 2, Neutrophil cytochrome b 91 kDa polypeptide, Superoxide-generating NADPH oxidase heavy chain subunit, gp91-1, gp91-phox, p22 phagocyte B-cytochrome) is encoded by the CYBB (also known as NOX2) gene (Gene ID: 1536) in Human. Gp91phox is a multi-pass membrane glycoprotein of the NADPH oxidase family that contains three extracellular domains, six transmembrane domains, and four cytoplasmic domains. It contains four iron binding sites and has a ferric oxidoreductase domain that is localized in the amino acids 54-286. It is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide and plays an important role in the host defense function of phagocytic cells. It also functions as a voltage-gated proton channel that mediates the H+ currents of resting phagocytes. It can undergo polyubiquitination at lysine 48 by RNF145 that triggers its endoplasmic reticulum-associated degradation. Clone NL7 binds to an epitope in the C-terminal region, but it does not inhibit the generation of superoxide. Mutations in CYBB gene are known to cause granulomatous disease that is characterized by the inability of neutrophils and phagocytes to kill ingested leading to life-threatening bacterial and fungal infections. (Ref.: (Ref.: Burritt, JB., et al. (2003). J. Immunol. 170 (12); 6082-6089).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing PBS without azide
      Quality LevelMQ200
      Applications
      ApplicationAnti-gp91phox (NOX2), clone NL7, Cat. No. MABS2193, is a mouse monoclonal antibody that detects Cytochrome b-245 heavy chain (gp91phox) and is tested for use in Flow Cytometry, Inhibition Analysis, and Western Blotting.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Inhibition
      Application NotesInhibition Analysis: A representative lot detected gp91phox (NOX2) in Inhibition applications (Burritt, J.B., et. al. (2003). J Immunol. 170(12):6082-9).

      Western Blotting Analysis: A representative lot detected gp91phox (NOX2) in Western Blotting applications (Burritt, J.B., et. al. (2003). J Immunol. 170(12):6082-9).

      Flow Cytometry Analysis: A representative lot detected gp91phox (NOX2) in Flow Cytometry applications (Burritt, J.B., et. al. (2003). J Immunol. 170(12):6082-9).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenPurified human neutrophil flavocytochrome b588 (gp91phox).
      EpitopeC-terminal
      CloneNL7
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone NL7 is a mouse monoclonal antibody that detects gp91-phox/NOX2. It targets an epitope within 10 amino acids from the C-terminal, fourth cytoplasmic domain.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CYBB
      • NOX2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~91 kDa observed; 65.34 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Human PMN cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected gp91phox (NOX2) in Human PMN cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStorage and Handling Stable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/ thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2193-25UG 04065265872690