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06-1293 Anti-hCMT1 Antibody

Anti-hCMT1 Antibody is a Sheep Polyclonal Antibody for detection of hCMT1 also known as mRNA (guanine-7-)methyltransferase & has been validated in WB.

Anti-hCMT1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Binding Protein (RBP) Gene Symbol: DKFZp686H1252, EC 2.1.1.56 , KIAA0398, MET, OTTHUMP00000162509, RG7MT1, hCMT1, hCMT1c, hMet, hcm1p UniProt Number: O43148
06-1293
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBShSerumPolyclonal Antibody
Description
Catalogue Number06-1293
DescriptionAnti-hCMT1 Antibody
Alternate Names
  • RNA (guanine-7-) methyltransferase
  • mRNA (guanine-7-)methyltransferase
  • mRNA (guanine-N(7)-)-methyltransferase
  • mRNA cap methyltransferase
Background InformationhCMT1 or mRNA (guanine-N(7)-)-methyltransferase belongs to the mRNA cap methyltransferase family. It is widely expressed across various tissues and is located in the nucleus. hCMT1 fuctions as a mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. It also binds RNA containing 5'-terminal GpppC. hCMT1 interacts with importin alpha, leading to the stimulation of both RNA-binding and methyltransferase activity. Interaction with importin alpha and beta is required for its nuclear localization. Importin beta dissociating in response to RanGTP allows RNMT-importin alpha to bind RNA substrates. hCMT1 also interacts with elongating form of polymerase II and RNGTT.
References
Product Information
FormatSerum
Control
  • HeLa cell lysate
PresentationSheep polyclonal serum containing 0.05% sodium azide.
Applications
ApplicationAnti-hCMT1 Antibody is a Sheep Polyclonal Antibody for detection of hCMT1 also known as mRNA (guanine-7-)methyltransferase & has been validated in WB.
Key Applications
  • Western Blotting
Application NotesWestern Blot Analysis: A 1:1,000 dilution from a previous lot detected hCMT1 in 10 µg of HeLa cell lysate.
Biological Information
ImmunogenHis-tagged recombinant protein corresponding to full length hCMT1.
HostSheep
SpecificityThis antibody recognizes hCMT1.
Species Reactivity
  • Human
Species Reactivity NoteProven to react with human.
Mouse and rat (77% sequence homology).
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • DKFZp686H1252
  • EC 2.1.1.56
  • KIAA0398
  • MET
  • OTTHUMP00000162509
  • RG7MT1
  • hCMT1
  • hCMT1c
  • hMet
  • hcm1p
Purification MethodUnpurified
UniProt Number
UniProt SummaryFUNTION: mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC.
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
SUBUNIT STRUCTURE: Interacts with importin alpha, leading to stimulate both RNA-binding and methyltransferase activity. Interaction with importin alpha and beta is required for its nuclear localization, importin beta dissociating in response to RanGTP, allowing RNMT-importin alpha to bind RNA substrates. Interacts with elongating form of polymerase II and RNGTT.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Widely expressed.
SEQUENCE SIMILARITIES: Belongs to the mRNA cap methyltransferase family.
Molecular Weight~55 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected hCMT1 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
06-1293 04053252740763