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NA70 Anti-p14ARF (Ab-2) Mouse mAb (14PO2)

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NA70
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~14 kDa p14ARF protein in HeLa cells. Sold under license of U.S. Patent 6,482,929.
      Catalogue NumberNA70
      Brand Family Calbiochem®
      References
      ReferencesBates, S., et al. 1998. Nature 395, 124.
      Product Information
      DeclarationSold under license of U.S. Patent 6,482,929.
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
      Negative controlNormal sheep serum
      Positive controlHeLa cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Application NotesImmnunoblotting (2-4 µg/ml)
      Immunofluorescence (0.5 µg/ml)
      Immunoprecipitation (2 µg/mg lysate)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, human p14ARF (p16β)
      ImmunogenHuman
      Clone14PO2
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      NA70 0

      Documentation

      Anti-p14ARF (Ab-2) Mouse mAb (14PO2) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-p14ARF (Ab-2) Mouse mAb (14PO2) Certificates of Analysis

      TitleLot Number
      NA70

      References

      Reference overview
      Bates, S., et al. 1998. Nature 395, 124.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-September-2007 RFH
      ApplicationImmnunoblotting (2-4 µg/ml)
      Immunofluorescence (0.5 µg/ml)
      Immunoprecipitation (2 µg/mg lysate)
      DescriptionPurified mouse monoclonal generated by immunizing BALB/c mice with the specified immunogen and fusing splonocytes with mouse myeloma cells. Recognizes the ~14 kDa p14ARF protein.
      BackgroundThe INK4α-ARF locus encodes two unrelated proteins both of which function in tumor suppression. p14ARF arrests the cell cycle in a p53-dependent manner. It binds to mdm2 and promotes the rapid degradation of mdm2 protein. This interaction is mediated by the exon 1-β-encoded N-terminal domain of p14ARF and a C-terminal region of mdm2. ARF-mediated mdm2 degradation is associated with mdm2 modification and concurrent p53 stabilization and accumulation. Deletion of the ARF-INK4α locus simultaneously impairs both the INK4α-cyclin D/cdk4-RB and the ARF-mdm2-p53 pathways.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant, human p14ARF (p16β)
      Clone14PO2
      IsotypeIgG₁
      Specieshuman, not mouse, not rat
      Positive controlHeLa cells
      Negative controlNormal sheep serum
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesBates, S., et al. 1998. Nature 395, 124.