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559322 Anti-p38/SAPK 2a Mouse mAb (20B11)

Anti-p38/SAPK 2a Mouse mAb (20B11) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-SAPK2a, Anti-p38α

559322
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~38 kDa SAPK2a protein. Does not recognize Mxi2.
      Catalogue Number559322
      Brand Family Calbiochem®
      SynonymsAnti-SAPK2a, Anti-p38α
      Application Data
      Detection of human p38/SAPK 2a by immunoblotting. Samples: Whole cell lysates from serum starved A431 cells (lane 1), SW480 cells (lane 2), SW620 cells (lane 3), HT29 cells (lane 4), MCF7 cells (lane 5), MDA-MB231 cells (lane 6), and T47D cells (lane 7). Primary antibody: Anti-p38/SAPK 2a Mouse mAb (20B11) (Cat. No. 559322) (0.5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLyophilized
      FormulationLyophilized from 2X PBS, PEG, sucrose.
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (0.05 µg/ml)
      Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
      Application CommentsRecognizes SAPK2α in human and mouse. Does not cross-react with Mxi2. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids near the N-terminus of human p38/SAPK2α, conjugated to KLH
      ImmunogenHuman
      Clone20B11
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute with 1 ml H₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solution.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      559322 0

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-August-2007 RFH
      SynonymsAnti-SAPK2a, Anti-p38α
      ApplicationELISA (0.05 µg/ml)
      Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
      Application Data
      Detection of human p38/SAPK 2a by immunoblotting. Samples: Whole cell lysates from serum starved A431 cells (lane 1), SW480 cells (lane 2), SW620 cells (lane 3), HT29 cells (lane 4), MCF7 cells (lane 5), MDA-MB231 cells (lane 6), and T47D cells (lane 7). Primary antibody: Anti-p38/SAPK 2a Mouse mAb (20B11) (Cat. No. 559322) (0.5 µg/ml). Detection: chemiluminescence.
      DescriptionMouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the ~38 kDa p38/SAPK2α protein.
      HostMouse
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids near the N-terminus of human p38/SAPK2α, conjugated to KLH
      Clone20B11
      IsotypeIgG₁
      Specieshuman, mouse
      FormLyophilized
      FormulationLyophilized from 2X PBS, PEG, sucrose.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsRecognizes SAPK2α in human and mouse. Does not cross-react with Mxi2. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute with 1 ml H₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solution.
      Toxicity Standard Handling