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MABT1333-25UL Anti-pan-Actin Antibody, clone 2A3

Anti-pan-Actin, clone 2A3, Cat. No. MABT1333, is a mouse monoclonal antibody that detects all forms of actin and has been tested for use in Western Blotting.

Anti-pan-Actin Antibody, clone 2A3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: ACTA1, ACTA2, ACTB, ACTC1, ACTG1, ACTG2 UniProt Number: P60709
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MABT1333-25UL
25 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, Yeast, Mk, H, Ht, Dr, RWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT1333-25UL
      DescriptionAnti-pan-Actin Antibody, clone 2A3
      Alternate Names
      • Skeletal muscle alpha-actin
      • Smooth muscle alpha-actin
      • Cytoplasmic beta-actin
      • Cardiac muscle alpha-actin
      • Cytoplasmic gamma-actin
      • Smooth muscle gamma-actin
      Background InformationActin is a multi-functional cytoskeletal protein that serves as the basic building block of cytoskeletal microfilaments. Six different actin types have been reported. Skeletal muscle alpha-actin (UniProt: P68133) is encoded by the ACTA1 gene, smooth muscle alpha-actin (UniProt: P62736) by the ACTA2 gene, cytoplasmic beta-actin (UniProt: P60709) by the ACTB gene, cardiac muscle alpha-actin (UniProt: P68032) by the ACTC1 gene, cytoplasmic gamma-actin (UniProt: P63261) by the ACTG1 gene, and smooth muscle gamma-actin (UniProt: P63267) by the ACTG2 (also known as ACTA3) gene. Although actins show about 90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic beta and gamma actins are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt. This pan-actin antibody detects all isoforms actin.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-pan-Actin, clone 2A3, Cat. No. MABT1333, is a mouse monoclonal antibody that detects all forms of actin and has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:50 dilution from a representative lot detected Actins in various species samples (e.g. Human, rat, mouse, monkey, Hamster, Drosophila, Yeast) (Courtesy of Martina Mitterhuber, M.sc and Egon Ogris, M.D., Medical University of Vienna, Austria).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a fragment from the C-terminal region of human Actin.
      Clone2A3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2A3 detects all forms of actin in multiple species. It targets an epitope with in the C-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Yeast
      • Monkey
      • Human
      • Hamster
      • Drosophila
      • Rat
      Species Reactivity NoteHuman, Monkey, Mouse, Rat, Yeast, Drosophila, Hamster.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ACTA1
      • ACTA2
      • ACTB
      • ACTC1
      • ACTG1
      • ACTG2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~42 kDa observed; 41.74 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Actins in HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT1333-25UL 04054839557965