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04-078 Anti-phospho-Androgen Receptor (Ser81) Antibody, rabbit monoclonal

Anti-phospho-Androgen Receptor (Ser81) Antibody detects level of phospho-Androgen Receptor (Ser81) & has been published & validated for use in WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: AR, AIS, DHTR, SMAX1, NR3C4, TFM, OTTHUMP00000061928, KD, HUMARA, SBMA UniProt Number: P10275
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04-078
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-078
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Androgen Receptor (Ser81) Antibody, rabbit monoclonal
      References
      Product Information
      FormatCulture Supernatant
      Presentation100μl of cell culture supernatant with 0.1% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho-Androgen Receptor (Ser81) Antibody detects level of phospho-Androgen Receptor (Ser81) & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenA synthetic phospho-peptide surrounding Ser81 of human Androgen Receptor.
      HostRabbit
      SpecificityAndrogen Receptor when phosphorylated on Ser81.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described.
      Gene Symbol
      • AR
      • AIS
      • DHTR
      • SMAX1
      • NR3C4
      • TFM
      • OTTHUMP00000061928
      • KD
      • HUMARA
      • SBMA
      Modifications
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P10275 # The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Activated, but not phosphorylated, by HIPK3.
      SIZE: 919 amino acids; 98989 Da
      SUBUNIT: Binds DNA as a homodimer. Part of a ternary complex containing AR, EFCAB6/DJBP and PARK7. Interacts with HIPK3 in the presence of androgen. The ligand binding domain interacts with MYST2/HBO1 in the presence of dihydrotestosterone. Interacts with EFCAB6/DJBP, PELP1, PQBP1, RANBP9, SPDEF, SRA1, TGFB1I1, ZNF318 and RREB1. The AR N-terminal poly-Gln region binds Ran resulting in enhancement of AR-mediated transactivation. Ran-binding decreases as the poly-Gln length increases. Interacts with ZMIZ1/ZIMP10 and ZMIZ2/ZMIP7 which both enhance its transactivation activity. Interacts with SLC30A9 (By similarity).
      SUBCELLULAR LOCATION: Nucleus.
      DOMAIN: SwissProt: P10275 Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain. Interaction with RANBP9 is mediated by both the N-terminal domain and the DNA-binding domain. Interaction with EFCAB6/DJBP is mediated by the DNA-binding domain.
      PTM: Sumoylated on Lys-386 (major) and Lys-520. & Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-534 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression.
      DISEASE: SwissProt: P10275 # Defects in AR are the cause of androgen insensibility syndrome (AIS) [MIM:300068]; previously known as testicular feminization syndrome (TFM). It can be complete (CAIS) when external genitalia are phenotypically female; or partial (PAIS) when external genitalia are substantively ambiguous or mild (MAIS) when external genitalia are normal male or nearly so. & Defects in AR are the cause of X-linked spinal and bulbar muscular atrophy (SBMA) [MIM:313200]; also known as Kennedy disease. In SBMA patients the number of Gln ranges from 40 to 52. Longer expansions result in earlier onset and more severe clinical manifestations of the disease. & Defects in AR may play a role in metastatic prostate cancer. The mutated receptor stimulates prostate growth and metastases development despite of androgen ablation. This treatment can reduce primary and metastatic lesions probably by inducing apoptosis of tumor cells when they express the wild-type receptor. & Defects in AR may be the cause of infertility male syndrome [MIM:308370]; also called androgen insensitivity. It is characterized by azoospermia, elevated testosterone and luteinizing hormone plasma levels and an abnormal androgen receptor. & Defects in AR are the cause of Reifenstein syndrome [MIM:312300]; also known as partial androgen insensitivity. The features of this form of male pseudohermaphroditism are hypospadias, hypogonadism, gynecomastia, normal XY karyotype, and a pedigree pattern consistent with X-linked recessive inheritance.
      SIMILARITY: Belongs to the nuclear hormone receptor family. NR3 subfamily. & Contains 1 nuclear receptor DNA-binding domain.
      MISCELLANEOUS: In the absence of ligand, steroid hormone receptors are thought to be weakly associated with nuclear components; hormone binding greatly increases receptor affinity. The hormone- receptor complex appears to recognize discrete DNA sequences upstream of transcriptional start sites. & Transcriptional activity is enhanced by binding to RANBP9. & The level of tyrosine phosphorylation may serve as a diagnostic tool to predict patient outcome in response to hormone-ablation therapy. Inhibition of tyrosine phosphorylation may be an effective intervention target for hormone-refractory prostate cancer.
      Molecular Weight99 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      04-078 04053252329401

      Documentation

      Required Licenses

      Title
      PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

      Anti-phospho-Androgen Receptor (Ser81) Antibody, rabbit monoclonal SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-Androgen Receptor (Ser81) Antibody, rabbit monoclonal Certificates of Analysis

      TitleLot Number
      Anti-phospho-Androgen Receptor (Ser81) (Rabbit monoclonal IgG) - 2147294 2147294
      Anti-phospho-Androgen Receptor (Ser81) (Rabbit monoclonal IgG) - 2370697 2370697
      Anti-phospho-Androgen Receptor (Ser81) (Rabbit monoclonal IgG) - 2433402 2433402
      Anti-phospho-Androgen Receptor (Ser81) (Rabbit monoclonal IgG) - 2023289 2023289
      Anti-phospho-Androgen Receptor (Ser81) (Rabbit monoclonal IgG) 3099531
      Anti-phospho-Androgen Receptor (Ser81) (Rabbit monoclonal IgG) 2922427
      Anti-phospho-Androgen Receptor (Ser81) (Rabbit monoclonal IgG) - 2197517 2197517
      Anti-phospho-Androgen Receptor (Ser81) (Rabbit monoclonal IgG) - 2212018 2212018
      Anti-phospho-Androgen Receptor (Ser81) (Rabbit monoclonal IgG) - 2275588 2275588
      Anti-phospho-Androgen Receptor (Ser81) (Rabbit monoclonal IgG) -2491157 2491157
      Show more

      References

      Reference overviewPub Med ID
      Androgen receptor and prostate apoptosis response factor-4 target the c-FLIP gene to determine survival and apoptosis in the prostate gland
      Gao, Shen, et al
      J Mol Endocrinol, 36:463-83 (2006)  2006

      		16720717 16720717
      Elevated E2F1 inhibits transcription of the androgen receptor in metastatic hormone-resistant prostate cancer
      Davis, Joanne N, et al
      Cancer Res, 66:11897-906 (2006)  2006

      		17178887 17178887

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies