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ABE12 Anti-phospho CARM1 (Ser228) Antibody

Anti-phospho CARM1 (Ser228) Antibody is a Rabbit Polyclonal Antibody for detection of phospho CARM1 (Ser228) also known as Protein arginine N-methyltransferase 4 & has been validated in WB.

Anti-phospho CARM1 (Ser228) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology, Histones Gene Symbol: CARM1, PRMT4 UniProt Number: Q86X55
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ABE12
200 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Eq, B, CaWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE12
      DescriptionAnti-phospho CARM1 (Ser228) Antibody
      Alternate Names
      • Protein arginine N-methyltransferase 4
      • coactivator-associated arginine methyltransferase 1
      • coactivator-associated arginine methyltransferase-1
      Background InformationCARM1 (coactivator-associated arginine methyltransferase 1) is an arginine N-methyltransferase that catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to arginine residues. This protein-arginine methyltransferase that works in conjunction with p300 and GRIP1 to enhance transcription. CARM1 methylation targets include histone H3 at Arg17 and p300 at Arg2142. CARM1 is recruited to promoters upon gene activation together with histone acetyltransferases from the p300 and p160 families. During monocyte inflammatory stimulation, CARM1 acts together with p300 as a coactivator for NF-kB. It is responsible for moving cells toward the inner cell mass in developing blastocysts. CARM1 also plays an important role in androgen receptors and may play a role in prostate cancer progression.
      References
      Product Information
      FormatAffinity Purified
      Control
      • EGF treated and untreated A431 cell lysates
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho CARM1 (Ser228) Antibody is a Rabbit Polyclonal Antibody for detection of phospho CARM1 (Ser228) also known as Protein arginine N-methyltransferase 4 & has been validated in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human CARM1 phosphorylated at Ser228.
      EpitopePhosphorylated Ser228
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes CARM1 phosphorylated at Ser228.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Horse
      • Bovine
      • Canine
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse, Rat, Equine, Bovine, and Canine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryProtein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation (Frankel et al., 2002 [PubMed 11724789]).[supplied by OMIM].
      Gene Symbol
      • CARM1
      • PRMT4
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' and activates transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.

      CATALYTIC ACTIVITY: S-adenosyl-L-methionine + arginine-[histone] = S-adenosyl-L-homocysteine + N(omega)-methyl-arginine-[histone].

      SUBUNIT STRUCTURE: Homodimer Probable. Interacts with the C-terminus of NCOA2/GRIP1, NCO3/ACTR and NCOA1/SRC1. Part of a complex consisting of CARM1, EP300/P300 and NCOA2/GRIP1. Interacts with FLII, TP53, myogenic factor MEF2, EP300/P300, CREBBP and CTNNB1. Interacts with SNRPC (By similarity). Interacts with NR1H4. Interacts with RELA. Interacts with HTLV-1 Tax-1.

      SUBCELLULAR LOCATION: Nucleus. Cytoplasm

      TISSUE SPECIFICTY: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.

      MISCELLANEOUS: Methylation of H3-R17 by CARM1 is stimulated by preacetylation of H3-K18/H3-K23 by EP300 and blocked by citrullination of H3-R17 by PADI4 (By similarity).

      SEQUENCE SIMILARITIES: Belongs to the protein arginine N-methyltransferase family.
      Molecular Weight ~63 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in EGF treated and untreated A431 cell lysates.

      Western Blot Analysis: 2 µg/mL of this antibody detected CARM1 on 10 µg of EGF treated and untreated A431 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE12 04053252415319

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies