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07-833 Anti-phospho-Gab1 (Tyr627) Antibody

This Anti-phospho-Gab1 (Tyr627) Antibody is validated for use in WB for the detection of phospho-Gab1 (Tyr627).

Anti-phospho-Gab1 (Tyr627) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: MAP Kinases Gene Symbol: GAB1 UniProt Number: Q13480
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07-833
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-833
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Gab1 (Tyr627) Antibody
      Alternate Names
      • GAB1
      • GRB2-associated binding protein 1
      References
      Product Information
      FormatAffinity Purified
      Presentation100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-phospho-Gab1 (Tyr627) Antibody is validated for use in WB for the detection of phospho-Gab1 (Tyr627).
      Key Applications
      • Western Blotting
      Application NotesImmunoblot Analysis: 1:1000 dilutions of this lot detected phospho-Gab1 (Tyr627) in RIPA lysate of PDGF treated NIH3T3 cells.
      Biological Information
      ImmunogenPeptide corresponding to amino acid region encompassing the human and mouse phospho-Gab1 (Tyr627)
      HostRabbit
      SpecificityGab1 phosphorylated on Tyrosine residue 627.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NotePredicted to cross-react with human based on 100% sequence homology, but have not been tested.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. The encoded protein is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • GAB1
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13480 # Probably involved in EGF and insulin receptor signaling.
      SIZE: 694 amino acids; 76616 Da
      SUBUNIT: Interacts with GRB2 and with other SH2-containing proteins. Interacts with phosphorylated LAT2.
      PTM: Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains.
      SIMILARITY: SwissProt: Q13480 ## Belongs to the GAB family. & Contains 1 PH domain.
      Molecular Weight115 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-833 04053252469459

      Documentation

      Anti-phospho-Gab1 (Tyr627) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-Gab1 (Tyr627) Antibody Certificates of Analysis

      TitleLot Number
      Anti-phospho-Gab1 (Tyr627) (Affinity purified Rabbit polyclonal IgG) 3115880
      Anti-phospho-Gab1 (Tyr627) (Affinity purified Rabbit polyclonal IgG) - DAM1465948 DAM1465948
      Anti-phospho-Gab1 (Tyr627) - 1988572 1988572
      Anti-phospho-Gab1 (Tyr627) - DAM1413923 DAM1413923
      anti-phospho-Gab1 (Tyr627) - 0604027241 0604027241
      anti-phospho-Gab1 (Tyr627) - 0604027242 0604027242
      anti-phospho-Gab1 (Tyr627) - 0610042766 0610042766
      anti-phospho-Gab1 (Tyr627) - 0612047427 0612047427
      anti-phospho-Gab1 (Tyr627) - JBC1378200 JBC1378200

      References

      Reference overviewPub Med ID
      Hyaluronan-mediated CD44 interaction with RhoGEF and Rho kinase promotes Grb2-associated binder-1 phosphorylation and phosphatidylinositol 3-kinase signaling leading to cytokine (macrophage-colony stimulating factor) production and breast tumor progressio
      Bourguignon, Lilly Y W, et al
      J Biol Chem, 278:29420-34 (2003)  2003

      		12748184 12748184
      Roles of cell-cell adhesion-dependent tyrosine phosphorylation of Gab-1
      Shinohara, M, et al
      J Biol Chem, 276:18941-6 (2001)  2001

      		11262408 11262408
      Induction of epithelial tubules by growth factor HGF depends on the STAT pathway
      Boccaccio, C, et al
      Nature, 391:285-8 (1998)  1998

      		9440692 9440692
      A Grb2-associated docking protein in EGF- and insulin-receptor signalling
      Holgado-Madruga, M, et al
      Nature, 379:560-4 (1996)  1996

      		8596638 8596638

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies