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ABE153 Anti-phospho Histone H3.3 Antibody (Thr32)

Detect Histone H3 using this rabbit polyclonal antibody, Anti-phospho Histone H3.3 Antibody (Thr32) validated for use in western blotting, IP & Dot Blot.

Anti-phospho Histone H3.3 Antibody (Thr32) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: H3F3A, H3.3A, H3F3, H3F3B, H3.3B UniProt Number: P84243
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ABE153
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IP, DBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE153
      DescriptionAnti-phospho Histone H3.3 Antibody (Thr32)
      Alternate Names
      • Histone H3.3
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3.3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Untreated and Colcemid-treated HeLa cell lysates, and in Histone H3 recombinant protein
      PresentationPurified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Histone H3 using this rabbit polyclonal antibody, Anti-phospho Histone H3.3 Antibody (Thr32) validated for use in western blotting, IP & Dot Blot.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Dot Blot
      Application NotesDot Blot (Specificity) Analysis: 0.2 µg/mL of this antibody detected phospho Histone H3.3 (Thr32), but not non-phospho Histone H3 (Thr32), or other peptides corresponding to modified histones.

      Immunoprecipitation Analysis: A representative lot detected phospho Histone H3.3 (Thr32) in IP.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a region near the N-terminus of human Histone H3.3 phosphorylated at Thr32.
      EpitopeNear N-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes a region near the N-terminus of Histone H3.3 phosphorylated at Thr32. This antibody demonstrates specificity for phospho Histone H3.3 (Thr32), but does not cross react with non-phospho Histone H3.3 (Thr32) or any other Histone proteins.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • H3F3A
      • H3.3A
      • H3F3
      • H3F3B
      • H3.3B
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~17 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in untreated and Colcemid-treated HeLa cell lysates, and in Histone H3 recombinant protein.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected phospho Histone H3.3 (Thr32) in 10 µg of Colcemid-treated HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE153 04053252909344