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ABN58 Anti-phospho MAP1B (Thr1265) Antibody

Anti-phospho MAP1B (Thr1265) Antibody is an antibody against phospho MAP1B (Thr1265) for use in WB, IH & IC.

Anti-phospho MAP1B (Thr1265) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neuronal & Glial Markers, Neurofilament & Neuron Metabolism Gene Symbol: Map1b, Mtap1b, Mtap5 UniProt Number: P14873
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ABN58
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, MWB, IHC, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN58
      DescriptionAnti-phospho MAP1B (Thr1265) Antibody
      Alternate Names
      • Microtubule-associated protein 1B
      • MAP-1B
      • MAP1(X)
      • MAP1.2
      • MAP1 light chain LC1
      Background InformationMicrotubule-associated protein 1B (MAP1B) is neuron-specific and is thought to play a part in controlling the neuronal cytoskeleton. MAP1B is made up of heavy and light chains and is involved in the microtubule and actin filament association. MAP1B may have an important role in axon formation by supporting microtubule dynamics and controlling cytoskeletal arrangement. MAP1B has also been associated with Netrin 1 signaling.
      References
      Product Information
      FormatAffinity Purified
      Control
      • S1 rat brain tissue lysate
      PresentationPurified rabbit polyclonal in PBS containing 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho MAP1B (Thr1265) Antibody is an antibody against phospho MAP1B (Thr1265) for use in WB, IH & IC.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunocytochemistry
      Application NotesImmunohistochemistry Analysis: 1:600 dilution from a representative lot detected MAP1B in embryonic (E12) rat spinal cord tissue. (Image courtesy of Dr. Phillip R. Gordon-Weeks, King's College London.)

      Immunofluorescence Analysis: A representative lot was used by an independent laboratory in IF. (Trivedi, N., et al. (2005). Journal of Cell Science. 118:993-1005.)
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to mouse MAP1B phosphorylated at Thr1265.
      EpitopePhosphorylated Thr1265
      HostRabbit
      SpecificityThis antibody recognizes MAP1B phosphorylated at Thr1265.
      Species Reactivity
      • Rat
      • Mouse
      Species Reactivity NoteDemonstrated to react with Rat. Predicted to react with Mouse based on immunogen design. Other homologies: Human (91% sequence homology).
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • Map1b
      • Mtap1b
      • Mtap5
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: The function of brain MAPS is essentially unknown. Phosphorylated MAP1B may play a role in the cytoskeletal changes that accompany neurite extension. Possibly MAP1B Binds to at least two tubulin subunits in the polymer, and this bridging of subunits might be involved in nucleating microtubule polymerization and in stabilizing microtubules.

      SUBUNIT STRUCTURE: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. LC1 interacts with the amino-terminal region of MAP1B. Interacts with ANP32A and TIAM2. Interacts (via C-terminus) with GAN (via Kelch domains).

      SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton Probable.

      DOMAIN: Has a highly basic region with many copies of the sequence KKEE and KKEI/V, repeated but not at fixed intervals, which is responsible for the binding of MAP1B to microtubules.

      PTM: LC1 is coexpressed with MAP1B. It is a polypeptide generated from MAP1B by proteolytic processing. It is free to associate with both MAP1A and MAP1B. It interacts with the N-terminal region of MAP1B.

      Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity).

      SEQUENCE SIMLARITIES: Belongs to the MAP1 family.
      Molecular Weight~300 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in S1 rat brain tissue lysate.

      Western Blot Analysis: 1:1,000 dilution of this antibody detected MAP1B on 10 µg of S1 rat brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN58 04053252292422

      Documentation

      Anti-phospho MAP1B (Thr1265) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho MAP1B (Thr1265) Antibody Certificates of Analysis

      TitleLot Number
      Anti-phospho MAP1B (Thr1265) - 3443357 3443357
      Anti-phospho MAP1B (Thr1265) - 3860255 3860255
      Anti-phospho MAP1B (Thr1265) - 4153603 4153603
      Anti-phospho MAP1B (Thr1265) -2615083 2615083
      Anti-phospho MAP1B (Thr1265) -2887489 2887489
      Anti-phospho MAP1B (Thr1265) Polyclonal Antibody 3011367
      Anti-phospho MAP1B (Thr1265) Polyclonal Antibody 3140080