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ABN2251-100UG Anti-phospho NMDAR2A (Ser1459)

Anti-phospho NMDAR2A (Ser1459), Cat. No. ABN2251, is a rabbit polyclonal antibody that detects NMDAR2A phosphorylated on Serine 1459 and is tested for use in Immunocytochemistry and Western Blotting.

ABN2251-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABN2251-100UG
      DescriptionAnti-phospho NMDAR2A (Ser1459)
      Alternate Names
      • Glutamate receptor ionotropic NMDA 2A
      • GluN2A
      • Glutamate [NMDA] receptor subunit epsilon-1
      • N-methyl D-aspartate receptor subtype 2A
      • NR2A
      • hNR2A
      Background InformationGlutamate receptor ionotropic, NMDA2A (UniProt: Q12879; also known as GluN2A, Glutamate [NMDA] receptor subunit epsilon-1, N-methyl D-aspartate receptor subtype 2A, NMDAR2A, NR2A, hNR2A) is encoded by the GRIN2A (also known as NMDAR2A) gene (Gene ID: 2903) in human. NMDA receptor complexes are ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. They are involved in long-term potentiation and neuronal plasticity and regulate the number of nerve cells during development. However, over-stimulation NMDA receptors can lead to neuronal degeneration. NMDAR2A is a multi-pass membrane glycoprotein that is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off. NMDAR2A exists as a heterotetramer and the heterotetrameric channels are composed of two zeta subunits (GRIN1) and two epsilon subunits (GRIN2A, GRIN2B, GRIN2C or GRIN2D) (in vitro). It can also form heterotetrameric channels that contain at least one zeta subunit (GRIN1), at least one epsilon subunit, plus GRIN3A or GRIN3B. In vivo, the subunit composition may depend on the expression levels of the different subunits. These heterotetrameric, ligand-gated ion channels display high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by magnesium ions. It has been shown that channels containing GRIN1 and GRIN2A have higher sensitivity to glutamate and faster kinetics than channels formed by GRIN1 and GRIN2B. Mutations in GRIN2A gene are known to cause epilepsy, speech disorders, and mental retardation. Two isoforms of NMDAR2A have ben described that are produced by alternative splicing. (Ref.: Hess, SD. et al. (1996). J. Pharmacol. Exp. Therap. 278(2); 808-816).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing PBS, pH 7.4, containing no preservatives.
      Quality LevelMQ200
      Applications
      ApplicationAnti-phospho NMDAR2A (Ser1459), Cat. No. ABN2251, is a rabbit polyclonal antibody that detects NMDAR2A phosphorylated on Serine 1459 and is tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesTested applications

      Western Blotting Analysis: A representative lot detected phospho NMDAR2A (Ser1459) in Western Blotting applications (Wyklicky, H., et. al. (1988). Wien Klin Wochenschr. 100(7):208-10).

      Immunocytochemistry Analysis: A representative lot detected phospho NMDAR2A (Ser1459) in Immunocytochemistry applications (Wyklicky, H., et. al. (1988). Wien Klin Wochenschr. 100(7):208-10).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenA peptide corresponding to 176 amino acids, phosphorylated on Serine 1459, from the C-terminal region of Human NMDAR2A.
      EpitopeC-terminal
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects Phospho-NMDAR2A (Ser1459).
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GRIN2A
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~200 kDa observed; 165.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysates from wild-type HEK293T cells.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NMDAR2A phosphorylated on serine 1459 in lysate from HEK293T cells, but not in lysates from cells transfected with S1459A NMDAR2A.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN2251-100UG 04065270172426