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MABS2269-100UG Anti-phospho-PD-1 (Tyr248) Antibody, clone 407.6G12

Anti-phospho-PD-1 (Tyr248), clone 407.6G12, Cat. No. MABS2269, is a mouse monoclonal antibody that detects PD-1 (CD279) and is tested for use in Western Blotting and Flow Cytometry.

MABS2269-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS2269-100UG
      DescriptionAnti-phospho-PD-1 (Tyr248) Antibody, clone 407.6G12
      Alternate Names
      • Programmed cell death protein 1
      • Protein PD-1
      • hPD-1
      • CD279
      Background InformationProgrammed cell death protein 1 (UniProt: Q15116; also known as Protein PD-1, hPD-1, CD279) is encoded by the PDCD1 (also known as PD1) gene (Gene ID: 5133) in human. PD-1 is a monomeric inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. PD-1 and its ligands, PD-L1 and PD-L2 play a key role in the maintenance of peripheral tolerance, a process by which the quiescence of autoreactive mature T cells is maintained. PD-1 is a single-pass type I membrane protein, synthesized with a signal peptide (aa 1-20), which is subsequently cleaved off. The mature form contains an extracellular domain (aa 24-170), a transmembrane domain (aa 171-191), and a cytoplasmic domain (aa 192-288). PD-1 also contains a single N-terminal immunoglobulin variable region (IgV) like domain, an ITIM motif (aa 221-226) and an ITSM motif ( aa 247-251). Tyrosine 248 falls in ITSM motif and located within the intracellular domain of PD-1 and, phosphorylation of PD-1 at Tyrosine 248 is a significant post-translational modification that influences its function. Src family kinases, such as Lck and Fyn, are involved in the initial phosphorylation of PD-1. Upon binding of PD-1 to its ligands (PD-L1 or PD-L2), phosphorylation at Tyr248 triggers downstream signaling pathways. PD-1 phosphorylation at Tyr248 promotes the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates de-phosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKC theta and CD247/CD3zeta. The phosphorylation at Tyr248 is crucial for the full inhibitory effect of PD-1 on T-cell activity. It contributes to the suppression of T-cell proliferation, cytokine production, and cytotoxic activity. Studies demonstrate that phosphorylation of the ITSM motif of PD-1 marks dysfunctional T cells that may be rescued with PD-1 blockade. Detection of phospho-PD-1 in TILs is a potential biomarker for PD-1 immunotherapy responses. (Ref.: Bu, X., et al. (2021). Cancer Immunol Res. 9(12):1465-1475).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-phospho-PD-1 (Tyr248), clone 407.6G12, Cat. No. MABS2269, is a mouse monoclonal antibody that detects PD-1 (CD279) and is tested for use in Western Blotting and Flow Cytometry.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected PD-1 (CD279) in Western Blotting application (Bu, X., et al. (2021). Cancer Immunol Res. 9(12):1465-1475).

      Flow Cytometry Analysis: A representative lot detected PD-1 (CD279) in Flow Cytometry application (Bu, X., et al. (2021). Cancer Immunol Res. 9(12):1465-1475).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 13 amino acids surrounding phospho-tyrosine 248 from the C-terminal half of human PD-1 (CD279).
      EpitopeC-terminal half
      Clone407.6G12
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 407.6G12 is a mouse monoclonal antibody that specifically detects PD-1 (CD279) phosphorylated on tyrosine 248.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Monkey based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PDCD1
      Modifications
      • Phosphorylation
      Purification MethodProtein G purified
      UniProt Number
      Molecular WeightTarget molecular weight ~55 kDa observed; 31.65 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from Jurkat cells treated with pervanadate (0.1 mM; 5 min.at 37C).

      Western Blotting Analysis (WB): A 1:1000 dilution of this antibody detected PD-1 phosphorylated on tyrosine 248 in lysate from Jurkat cells treated with pervanadate (0.1 mM; 5 min. at 37C), but not in lysate from untreated Jurkat cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2269-100UG 04065272163552