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559240 Anti-rSec6 Mouse mAb (9H5)

This Anti-rSec6 Mouse mAb (9H5) is validated for use in Immunoblotting, Immunofluorescence for the detection of rSec6.

Anti-rSec6 Mouse mAb (9H5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      B, Ca, Ch, Ht, H, Mk, M, Porcine, Rb, RMMonoclonal Antibody
      Description
      OverviewRecognizes the ~87 kDa rSec6 protein in PC12 cells.
      Catalogue Number559240
      Brand Family Calbiochem®
      Application Data
      Detection of pig rSec6 by immunoblotting. Sample: Whole cell lysate from ESK-4 cells. Primary antibody: Anti-rSec6 Mouse mAb (9H5) (Cat. No. 559240) (1:250). Detection: chemiluminescence.
      References
      ReferencesKee, Y., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 14438.
      Hsu, S.-C., et al. 1996. Neuron 17, 1209.
      TerBush, D.R., et al. 1995. J. Cell Biol. 130, 299.
      Product Information
      FormLiquid
      FormulationIn PBS, 0.1 mM PMSF, 50% glycerol, pH 7.2.
      Positive controlESK-4 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Application NotesImmunoblotting (4 µg/ml, chemiluminescence)
      Immunofluorescence (see comments)
      Application CommentsImmunofluorescence of PC12 cells using this antibody shows colocalization of rSec6 with vesicles and sites of exocytosis. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenrecombinant, rat rSec6 fused to a His•Tag® sequence
      ImmunogenRat
      Clone9H5
      HostMouse
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Canine
      • Chicken
      • Hamster
      • Human
      • Monkey
      • Mouse
      • Porcine
      • Rabbit
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      559240 0

      Documentation

      Anti-rSec6 Mouse mAb (9H5) SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Kee, Y., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 14438.
      Hsu, S.-C., et al. 1996. Neuron 17, 1209.
      TerBush, D.R., et al. 1995. J. Cell Biol. 130, 299.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-August-2007 RFH
      ApplicationImmunoblotting (4 µg/ml, chemiluminescence)
      Immunofluorescence (see comments)
      Application Data
      Detection of pig rSec6 by immunoblotting. Sample: Whole cell lysate from ESK-4 cells. Primary antibody: Anti-rSec6 Mouse mAb (9H5) (Cat. No. 559240) (1:250). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified mouse monoclonal antibody. Recognizes the ~87 kDa rSec6 protein.
      BackgroundrSec is a rat homolog of yeast Sec6p, a protein involved in transport between the Golgi apparatus and the plasma membrane. In yeast, Sec6p is part of a high molecular weight complex that includes Sec8p and Sec15p, that associates with the Ras-like GRP-binding protein Sec4p. An analogous 17S complex in rat brain contains 8 novel proteins, including rSec6 and rSec8. Membrane-associated rSec6/8 complex co-immuno-precipitates with syntaxin and is ubiquitously expressed, suggesting the complex is involved in the mammalian exocyst complex.
      HostMouse
      Immunogen speciesRat
      Immunogenrecombinant, rat rSec6 fused to a His•Tag® sequence
      Clone9H5
      IsotypeIgG
      Speciesbovine, canine, chicken, hamster, human, monkey, mouse, porcine, rabbit, rat
      Positive controlESK-4 cells
      FormLiquid
      FormulationIn PBS, 0.1 mM PMSF, 50% glycerol, pH 7.2.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsImmunofluorescence of PC12 cells using this antibody shows colocalization of rSec6 with vesicles and sites of exocytosis. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesKee, Y., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 14438.
      Hsu, S.-C., et al. 1996. Neuron 17, 1209.
      TerBush, D.R., et al. 1995. J. Cell Biol. 130, 299.