Millipore Sigma Vibrant Logo

05-678 Anti-ubiquityl-Histone H2A Antibody, clone E6C5

View Products on
200 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited Availability
Limited Availability
In Stock 
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers


      Contact Customer Service


      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, Mk, AmICC, WB, ChIPMPurifiedMonoclonal Antibody
      Catalogue Number05-678
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-ubiquityl-Histone H2A Antibody, clone E6C5
      Alternate Names
      • H2AUb
      • Histone H2A (ubiquityl)
      • H2A histone family, member R
      • histone 1
      • H2aa
      • histone H2A
      • histone cluster 1
      • H2aa
      • H2AK119Ub1
      Background InformationHistones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histones are modified post-translationally by acetylation, phosphorylation, methylation, and ubiquitination and these modifications regulate DNA transcription, repair, recombination, and replication. Ubiquitylation usually targets the substrate for degradation, although histones H2A and H2B are actually stabilized by a single ubiquitin conjugation. Histone ubiquitination has been correlated with DNA repair and transcription, cellular differentiation, cell cycle regulation, spermatogenesis, protein trafficking, and response to stress. Histone H2A is monoubiquitinated at Lys119 by the PRC-1L complex (Polycomb repressive complex 1-like), which includes Ring1, Ring2, Bmi1 and HPH2. Ubiquitinated H2A normally represents about 15% of H2A, but this value can be as high as 50% in active chromatin. Ubiquitinyl histone H2A has also been associated with transcriptional silencing of large chromatin regions and linked to Polycomb silencing so the function of ubiquitinated H2A remains undefined.
      Product Information
      HS Code3002 15 90
      • Acid extracts of HeLa cells.
      PresentationPurified mouse monoclonal IgM in buffer containing PBS with 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20ºC.
      Quality LevelMQ100
      ApplicationUse Anti-ubiquityl-Histone H2A Antibody, clone E6C5 (Mouse Monoclonal Antibody) has been published and validated for use in ChIP, ICC, WB to detect ubiquityl-Histone H2A also known as H2AUb, Histone H2A (ubiquityl), histone H2A.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesImmunocytochemistry:
      This antibody has been reported by an independent laboratory to detect ubiquityl-Histone H2A in cells fixed with either 3% formaldehyde/0.1% Triton X-100 or methanol (Vassilev, A. 1995).

      Chromatin Immunoprecipitation:
      Reported by an independent laboratory (Wang, H. 2004).
      Biological Information
      ImmunogenAMA (human epithelial amnion) cell residual nuclear pellet portions.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes and is specific for monoubiquityl-Histone H2A (Lys119), Mr ~25 kDa.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Monkey
      • Amphibian
      Species Reactivity NoteHuman, mouse, rat, monkey, and frog. Does not cross-react with army worm. Other species not tested.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. [provided by RefSeq].
      Gene Symbol
      • HIST1H2AA
      • H2AFR
      • bA317E16.2
      • Ubiquitylation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q96QV6 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 131 amino acids; 14234 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      PTM: The chromatin-associated form is phosphorylated on Thr-121 during mitosis (Probable). & Deiminated on Arg-4 in granulocytes upon calcium entry. & Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. & Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1. & Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity).
      SIMILARITY: SwissProt: Q96QV6 ## Belongs to the histone H2A family.
      Molecular Weight25 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on H2A in acid extracts from HeLa and 10T1/2 cells.

      Western Blot Analysis:
      A 1:500-1:2000 dilution of this lot detected ubiquityl-Histone H2A in acid extracts from HeLa and 10T1/2 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Catalogue Number GTIN
      05-678 04053252510571


      Anti-ubiquityl-Histone H2A Antibody, clone E6C5 SDS


      Safety Data Sheet (SDS) 

      Anti-ubiquityl-Histone H2A Antibody, clone E6C5 Certificates of Analysis

      TitleLot Number
      Anti-ubiquityl-Histone H2A, clone - 3229363 3229363
      Anti-ubiquityl-Histone H2A, clone -2551216 2551216
      Anti-ubiquityl-Histone H2A, clone E6C5 - 2430485 2430485
      Anti-ubiquityl-Histone H2A, clone E6C5 - 1970374 1970374
      Anti-ubiquityl-Histone H2A, clone E6C5 - 2019509 2019509
      Anti-ubiquityl-Histone H2A, clone E6C5 - 2041027 2041027
      Anti-ubiquityl-Histone H2A, clone E6C5 - 2073775 2073775
      Anti-ubiquityl-Histone H2A, clone E6C5 - 2239798 2239798
      Anti-ubiquityl-Histone H2A, clone E6C5 - 22424 22424
      Anti-ubiquityl-Histone H2A, clone E6C5 - 3018776 3018776


      Reference overviewApplicationSpeciesPub Med ID
      Epigenetic Regulation of Antibody Responses by the Histone H2A Deubiquitinase MYSM1.
      Jiang, XX; Chou, Y; Jones, L; Wang, T; Sanchez, S; Huang, XF; Zhang, L; Wang, C; Chen, SY
      Scientific reports  5  13755  2015

      Show Abstract
      26348977 26348977
      Impact of flanking chromosomal sequences on localization and silencing by the human non-coding RNA XIST.
      Kelsey, AD; Yang, C; Leung, D; Minks, J; Dixon-McDougall, T; Baldry, SE; Bogutz, AB; Lefebvre, L; Brown, CJ
      Genome biology  16  208  2015

      Show Abstract
      26429547 26429547
      The EBNA3 family of Epstein-Barr virus nuclear proteins associates with the USP46/USP12 deubiquitination complexes to regulate lymphoblastoid cell line growth.
      Ohashi, M; Holthaus, AM; Calderwood, MA; Lai, CY; Krastins, B; Sarracino, D; Johannsen, E
      PLoS pathogens  11  e1004822  2015

      Show Abstract
      25855980 25855980
      The specification and global reprogramming of histone epigenetic marks during gamete formation and early embryo development in C. elegans.
      Samson, M; Jow, MM; Wong, CC; Fitzpatrick, C; Aslanian, A; Saucedo, I; Estrada, R; Ito, T; Park, SK; Yates, JR; Chu, DS
      PLoS genetics  e1004588  2014

      Show Abstract
      Immunofluorescence25299455 25299455
      USP3 counteracts RNF168 via deubiquitinating H2A and γH2AX at lysine 13 and 15.
      Sharma, N; Zhu, Q; Wani, G; He, J; Wang, QE; Wani, AA
      Cell cycle (Georgetown, Tex.)  13  106-14  2014

      Show Abstract
      Western Blotting24196443 24196443
      The histone H2A deubiquitinase Usp16 regulates embryonic stem cell gene expression and lineage commitment.
      Yang, W; Lee, YH; Jones, AE; Woolnough, JL; Zhou, D; Dai, Q; Wu, Q; Giles, KE; Townes, TM; Wang, H
      Nature communications  5  3818  2014

      Show Abstract
      24784029 24784029
      BMI1 reprogrammes histone acetylation and enhances c-fos pathway via directly binding to Zmym3 in malignant myeloid progression.
      Shen, H; Chen, Z; Ding, X; Qi, X; Cen, J; Wang, Y; Yao, L; Chen, Y
      Journal of cellular and molecular medicine  18  1004-17  2014

      Show Abstract
      24571310 24571310
      Impact of human MLL/COMPASS and polycomb complexes on the DNA methylome.
      Putiri, EL; Tiedemann, RL; Liu, C; Choi, JH; Robertson, KD
      Oncotarget  5  6338-52  2014

      Show Abstract
      Western Blotting25071008 25071008
      Ubiquitin-specific peptidase 42 (USP42) functions to deubiquitylate histones and regulate transcriptional activity.
      Hock, AK; Vigneron, AM; Vousden, KH
      J Biol Chem  289  34862-70  2014

      Show Abstract
      Western Blotting25336640 25336640
      USP49 deubiquitinates histone H2B and regulates cotranscriptional pre-mRNA splicing.
      Zhang, Z; Jones, A; Joo, HY; Zhou, D; Cao, Y; Chen, S; Erdjument-Bromage, H; Renfrow, M; He, H; Tempst, P; Townes, TM; Giles, KE; Ma, L; Wang, H
      Genes & development  27  1581-95  2013

      Show Abstract
      23824326 23824326


      Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.
      Shaping Epigenetics Discovery - Epigenetics Product Selection Brochure

      Technical Info

      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics


      Histone Modifications

      Related Products & Applications

      Product Families


      Life Science Research > Antibodies and Assays > Primary Antibodies