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531370 BMP-2 Signaling Enhancer, A01 - Calbiochem

531370
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Overview

Replacement Information

Key Spec Table

Empirical Formula
C₂₂H₂₀ClF₃N₄O₃S

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      Glass bottle 10 mg
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      Description
      OverviewA cell-permeable phenylsulfonyl-piperazinyl compound that enhances BMP-2 associated signaling by selectively inhibiting Smurf1-mediated Smad1/5 ubiquitination and thereby prolonging the half life of Smad1 and 5. Directly and specifically blocks WW domains mediated Smurf1-Smad1/5 complex formation (Kd = 3.664 nM), but has no effect on Smurf2-Smad2 and Smurf2-Smad3 interaction. Increases Smad1/5 levels in cells pre-transfected wild type Smurf1, but not the C699A mutant Smurf1 lacking ubiquitin ligase activity. Does not affect the mRNA levels of Smad1 and Smad5. Also, has no effect on the level of Smad2/3, Smad4, ING2 and RhoA. Shown to potentiate BMP-2 induced osteoblastic differentiation of C2C12 cells (~ 2 µM in 48 h).

      Please note that the molecular weight for this compound is batch-specific due to variable water content.
      Catalogue Number531370
      Brand Family Calbiochem®
      Synonyms(4-((4-Chloro-3-(trifluoromethyl)phenyl)sulfonyl)-1-piperazinyl)(4-(5-methyl-1H-pyrazol-1-yl)phenyl)methanone, Smurf1 Inhibitor, A01
      References
      ReferencesCao, Y., et al. 2014. Sci. Rep. 4, 4965.
      Product Information
      FormYellow powder
      Hill FormulaC₂₂H₂₀ClF₃N₄O₃S
      Chemical formulaC₂₂H₂₀ClF₃N₄O₃S
      ReversibleY
      Quality LevelMQ100
      Applications
      Biological Information
      Primary Target Smurf1
      Purity≥97% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      BMP-2 Signaling Enhancer, A01 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Cao, Y., et al. 2014. Sci. Rep. 4, 4965.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision31-July-2014 JSW
      Synonyms(4-((4-Chloro-3-(trifluoromethyl)phenyl)sulfonyl)-1-piperazinyl)(4-(5-methyl-1H-pyrazol-1-yl)phenyl)methanone, Smurf1 Inhibitor, A01
      DescriptionA cell-permeable phenylsulfonyl-piperazinyl compound that enhances BMP-2 associated signaling by selectively inhibiting Smurf1-mediated Smad1/5 ubiquitination and thereby prolonging the half life of Smad1 and 5. Directly and specifically blocks WW domains mediated Smurf1-Smad1/5 complex formation (Kd = 3.664 nM), but has no effect on Smurf2-Smad2 and Smurf2-Smad3 interaction. Increases Smad1/5 levels in cells pre-transfected wild type Smurf1, but not the C699A mutant Smurf1 lacking ubiquitin ligase activity. Does not affect the mRNA levels of Smad1 and Smad5. Also, has no effect on the level of Smad2/3, Smad4, ING2 and RhoA. Shown to potentiate BMP-2 induced osteoblastic differentiation of C2C12 cells (~ 2 µM in 48 h).
      FormYellow powder
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₂₂H₂₀ClF₃N₄O₃S
      Purity≥97% by HPLC
      SolubilityDMSO (50 mg/ml)
      Storage +2°C to +8°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesCao, Y., et al. 2014. Sci. Rep. 4, 4965.