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SCT251 BioTracker BSH Activity Bioluminescent Probe


This BSH-activatable luciferin (BAL) probe provides a noninvasive optical readout-based method for quantifying BSH activity. Biological applications include measurement of BSH activity of pure enzyme, or diverse samples such as live bacteria, fecal samples, or through the use of in vivo noninvasive imaging.

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SCT251
1 mg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCT251
      DescriptionBioTracker BSH Activity Bioluminescent Probe
      OverviewReference
      Sci Adv. 2021, 7(6): eaaz9857.
      Alternate Names
      • Bile Salt Hydrolase probe
      • BSH probe
      • BSH bioluminescent probe
      • BSH cell and tissue probe
      Background InformationThe human gut microbiome and its role in both health and disease has been studied extensively, laying the foundation for its involvement in metabolism, nutrition, physiology, and immune function. Bile salt hydrolase (BSH), an enzyme produced by gut bacteria, cleaves an amide bond in conjugated bile acids that results in formation of glycine and taurine molecules as well as secondary bile acids. These are important in host lipid and glucose metabolism as well as gut microbe composition, providing necessary components for gastrointestinal microbe survival. Secondary bile acids also play a role in a variety of health conditions such as colon cancer and age-related diseases relevant to life-span. Increased BSH activity has positive benefits in a variety of areas such as reduced inflammation, reduced cholesterol levels, improved protection against colon cancer, reduction of urinary tract infections, and even improved symptoms for patients with Crohn’s disease. However, the mechanisms by which BSH function is regulated are poorly understood, and knowledge is limited by lack of noninvasive detection methods amenable to use within an intact gut microbiome environment.
      References
      Product Information
      Detection methodBioluminescence Luminometry
      FormatLyophilized solid
      PresentationLyophilized. Yellow solid.
      Quality LevelMQ100
      Applications
      Application
      This BSH-activatable luciferin (BAL) probe provides a noninvasive optical readout-based method for quantifying BSH activity. Biological applications include measurement of BSH activity of pure enzyme, or diverse samples such as live bacteria, fecal samples, or through the use of in vivo noninvasive imaging.
      Key Applications
      • Activity Assay
      • Cell Based Assays
      • Microbial monitoring
      • Enzyme Assays
      Application NotesBioluminescent enzyme activity measurement in cells and tissues
      Biological Information
      Molecular Weight653.89
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance
      Purity: ≥ 97% confirmed by HPLC, HNMR, LC-MS and elemental analysis. Molar Mass: 653.89 g/mol
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions
      Store BioTracker™ Bile Salt Hydrolase Bioluminescent Probe at -20°C, desiccated and protected from light
      Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCT251 04065268463642