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SCT078 BioTracker Green HOCl-CM2 Live Cell Dye

Off-on fluorescence probe for the specific detection of hypochlorous acid in Alheimer's disease studies.

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SCT078
1 mg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCT078
      DescriptionBioTracker Green HOCl-CM2 Live Cell Dye
      OverviewAlzheimer’s disease (AD) is a progressive neurological disorder characterized by the presence of amyloid-beta (Aβ) plagues and neurofibrillary tangles. AD is associated with generation of excessive reactive oxygen species (ROS) in the brain, which cause oxidative stress and inflammation. ROS production involves elevated levels of hypochlorous acid (HOCl) and can serve as a potential biomarker of AD pathogenesis along with amyloid plagues (1).

      BioTracker Green HOCl-CM2 Live Cell Dye is a switchable coumarin-morpholine (CM) off-on fluorescence probe for the specific detection of HOCl produced and proximally localized with amyloid plaques. The nonfluorescent thioamide probe undergoes regioselective transformation to fluorescent amide probe in the presence of HOCl with high selectivity and sensitivity (1). This probe has excellent cellular uptake and can penetrate the blood-brain barrier (BBB) enabling detection, imaging, and quantification of HOCl in vitro and in vivo (1).

      Spectral Properties
      Absorbance: 417 nm
      Emission: 485 nm

      Reference:
      1. Samanta, S., & Govindaraju, T. (2019). Unambiguous detection of elevated levels of hypochlorous acid in double transgenic AD mouse brain. ACS chemical neuroscience, 10(12), 4847-4853.
      Alternate Names
      • Live Cell Imaging Probe
      References
      Product Information
      Detection methodFluorescence
      PresentationLyophilized. Yellow solid.
      Applications
      ApplicationOff-on fluorescence probe for the specific detection of hypochlorous acid in Alheimer's disease studies.
      Key Applications
      • Cell Based Assays
      Application NotesLive cell fluorescent imaging
      Biological Information
      Molecular Weight346.1
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssurancePurity ≥ 98% confirmed by HPLC. Identification confirmed by HNMR, LC-MS, and elemental analysis.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20°C, desiccate and protect from light
      Note: Centrifuge vial briefly to collect contents at bottom of vial before opening
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCT078 04061841827948

      Documentation