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SCT262 BioTracker™ Mitochondrial FerroGreen Live Cell Probe

This probe is designed for the detection of mitochondrial ferrous iron in live cells by fluorescent microscopy or flow cytometry.

SCT262
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCT262
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      DescriptionBioTracker™ Mitochondrial FerroGreen Live Cell Probe
      OverviewSCT262 is a novel fluorescent probe for the detection of ferrous ion (Fe2+) in mitochondria where Fe-S clusters and heme proteins are synthesized, and enables live cell fluorescent imaging of intracellular Fe2+. The SCT262 probe has no chelating ability.

      Add 53 uL DMSO to the 50 ug probe in the tube to create 1mM stock solution. Dilute with HBSS to prepare 5uM working solution.

      Cells may be incubated for 30 mins in the working solution, after which the supernatant solution can be replaced with the desired treatment solution.


      SPECTRAL PROPERTIES
      λex: 505 nm
      λem: 535 nm
      Suitable for use with FITC/GFP laser or filter
      Alternate Names
      • mitochondrial iron live cell probe
      • mitochondrial iron probe
      Background InformationIron is thought to be the most abundant transition metal element in many organisms, participating in diverse physiological activities. Recently, free iron in living cells has received attention due to its significance related to cellular damage or death. Free iron exists in its stable redox states, ferrous ion (Fe2+) and ferric ion (Fe3+). In living cells, understanding the behavior of Fe2+ is important because of the intracellular reductive environment, metal porters and water solubility of Fe2+.
      SCT262 is a novel fluorescent probe for the detection of ferrous ion (Fe2+) in mitochondria where Fe-S clusters and heme proteins are synthesized. This probe enables live cell fluorescent imaging of intracellular Fe2+. SCT262 has no no chelating ability. Mitochondrial FerroGreen and Fe2+ react irreversibly, which is different from the detection principle of calcium-iron probes such as Fluo-3.
      References
      Product Information
      Detection methodFluorescence Flow cytometry
      FormatLyophilized solid
      PresentationLyophilized solid
      Quality LevelMQ100
      Applications
      ApplicationThis probe is designed for the detection of mitochondrial ferrous iron in live cells by fluorescent microscopy or flow cytometry.
      Key Applications
      • Cell Based Assays
      • Fluorescence
      • Flow Cytometry
      • Fluorescence Activated Cell Sorting (FACS)
      Application NotesLive cell probe for fluorescent detection of ferrous iron (Fe2+)in mitochondria by fluorescence microscopy or flow cytometry. SCT262 is optimized for the imaging of intracellular iron in live cell applications.
      IMPORTANT APPLICATION NOTE: Dissolve the lyophilized probe in in DMSO to make a 1 mM stock solution, and dilute with HBSS or serum-free medium to make a 5 uM immediately before staining. Use DMSO-based stock solution same day. Alternatively, probe dissolved in ethanol is stable at -20C for two weeks.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance
      Purity: ≥ 90% confirmed by HPLC.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore BioTracker™ Mitochondrial FerroGreen Live Cell Probe at -20 °C, protected from light.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCT262 04065269218159

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