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SCT214 BioTracker Orange-NHS Live Cell pH dye

Live Cell imaging probe which reversibly fluoresces in acidic pH, such as in lysosomes and endosomes of cells.

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SCT214
1 mg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCT214
      DescriptionBioTracker Orange-NHS Live Cell pH dye
      OverviewBioTracker Orange-NHS Live Cell pH dye is a chemical probe which reversibly fluoresces upon acidic pH. The probe is modified with N-hydroxysuccimide ester (NHS) which forms a covalent bond with primary amines just by mixing. It can be used for labeling proteins, antibodies and other molecules. For example, it can be applied for visualizing endocytosis of target protein on cell membrane by labeling antibodies that binds to the target protein. This probe binds to primary amines just by mixing. Thus it can be used to label antibodies and other proteins which have lysine residues. Its fluorescence intensity increases ~20-fold at an acidic pH.

      Spectral Properties
      Absorbance (max): 544 nm
      Emission (max): 565 nm (Orange)

      References
      Asanuma D et al. Acidic-pH-Activable Fluorescence Probes for Visualizing Exocytosis Dynamics. Angew. Chem Int Ed Engl. 2014 Jun 10; 53(24): 6085-9.
      Alternate Names
      • AcidiFluor™ ORANGE NHS
      References
      Product Information
      PresentationLyophilized.
      Quality LevelMQ200
      Applications
      ApplicationLive Cell imaging probe which reversibly fluoresces in acidic pH, such as in lysosomes and endosomes of cells.
      Key Applications
      • Cell Based Assays
      • Antibody Labeling
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssurancePurity: ≥ 80% (LC)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore BioTracker Orange-NHS Live Cell pH Dye at -20°C, desiccate and protect from light
      Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCT214 04061841697640