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208707 Ca2+/Calmodulin Kinase II, Rat Brain

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: CaM Kinase II

208707
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.



We are currently only offering Ca2+/Calmodulin Kinase II, α-Subunit, Rat, Recombinant, Spodoptera frugiperda (Cat. No. 208706) as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.






Native Ca2+/calmodulin kinase II from rat brain. A multi-functional calcium and calmodulin-dependent protein kinase that regulates a number of cellular functions including neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis. Reduced levels are reported following ischemia and neuronal injury. Consists of subunits of M.W. 50,000 and 58,000 - 60,000 in a 3:1 ratio.
Catalogue Number208707
Brand Family Calbiochem®
SynonymsCaM Kinase II
References
ReferencesTakeuchi, Y., et al. 2000. J. Neurochem. 74, 2557.
Enslen, H., and Soderling, T.R. 1994. J. Biol. Chem. 269, 20872.
Lisman, J. 1994. Trends Neurosci. 17, 406.
Bading, H., et al. 1993. Science 260, 181.
Machu, T.K., et al. 1993. J. Neurochem. 61, 375.
Shackelford, D.A., et al. 1993. J. Neurochem. 61, 738.
Hanson, P.I., and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.
Browning, M.D., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 1315.
Patton, B.L., et al. 1990. J. Biol. Chem. 265, 11204.
Kelly, P.T., and Shenolikar, S. 1987. Methods Enzymol. 139, 690.
Product Information
CAS number9026-43-1
Unit of DefinitionOne unit is defined as the amount of enzyme that will transfer 1.0 µmol phosphate to synapsin I per min at 30°C.
FormLiquid
FormulationIn 25 mM Tris-HCl, 10 mM DTT, 500 µM EDTA, 10 µM AEBSF, 100 µg/ml leupeptin, 50% glycerol, pH 7.5.
Applications
Biological Information
Purity≥95% by SDS-PAGE
Specific Activity≥1.0 µmol/min/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
208707 0

Documentation

Ca2+/Calmodulin Kinase II, Rat Brain Certificates of Analysis

TitleLot Number
208707

References

Reference overview
Takeuchi, Y., et al. 2000. J. Neurochem. 74, 2557.
Enslen, H., and Soderling, T.R. 1994. J. Biol. Chem. 269, 20872.
Lisman, J. 1994. Trends Neurosci. 17, 406.
Bading, H., et al. 1993. Science 260, 181.
Machu, T.K., et al. 1993. J. Neurochem. 61, 375.
Shackelford, D.A., et al. 1993. J. Neurochem. 61, 738.
Hanson, P.I., and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.
Browning, M.D., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 1315.
Patton, B.L., et al. 1990. J. Biol. Chem. 265, 11204.
Kelly, P.T., and Shenolikar, S. 1987. Methods Enzymol. 139, 690.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-August-2008 RFH
SynonymsCaM Kinase II
DescriptionNative Ca2+/calmodulin kinase II from rat brain. Multifunctional calcium and calmodulin dependent protein kinase that regulates a number of cellular functions including, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and calcium homeostasis. Reduced levels are reported following ischemia and neuronal injury. Consists of subunits of M.W. 50,000 and 58,000-60,000 in a 3:1 ratio. Phosphorylates a number of substrates including calcineurin, CRE-binding protein, IP3 receptor, intermediate filaments, microtubule-associated proteins, ribosomal protein S6, synapsin I, tyrosine hydroxylase, and tryptophan hydroxylase.
FormLiquid
FormulationIn 25 mM Tris-HCl, 10 mM DTT, 500 µM EDTA, 10 µM AEBSF, 100 µg/ml leupeptin, 50% glycerol, pH 7.5.
Recommended reaction conditions
Reaction Mixture: 50 mM HEPES, pH 7.4, 5 mM MgCl2, 0.3 mM CaCl2, 30 µg/ml calmodulin, 0.2 mg/ml synapsin I, 0.6 µg/ml CaM Kinase II, and 50 µM ATP (1-10 µCi of [γ-32P]ATP). Samples are typically incubated for 2 min at 22-30°C.
CAS number9026-43-1
Purity≥95% by SDS-PAGE
Specific activity≥1.0 µmol/min/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will transfer 1.0 µmol phosphate to synapsin I per min at 30°C.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesTakeuchi, Y., et al. 2000. J. Neurochem. 74, 2557.
Enslen, H., and Soderling, T.R. 1994. J. Biol. Chem. 269, 20872.
Lisman, J. 1994. Trends Neurosci. 17, 406.
Bading, H., et al. 1993. Science 260, 181.
Machu, T.K., et al. 1993. J. Neurochem. 61, 375.
Shackelford, D.A., et al. 1993. J. Neurochem. 61, 738.
Hanson, P.I., and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.
Browning, M.D., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 1315.
Patton, B.L., et al. 1990. J. Biol. Chem. 265, 11204.
Kelly, P.T., and Shenolikar, S. 1987. Methods Enzymol. 139, 690.