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208706 Ca2+/Calmodulin Kinase II, α-Subunit, Rat, Recombinant, Spodoptera frugiperda

Recombinant, rat Ca2+/calmodulin kinase II α-subunit consisting of amino acids 1-325 expressed in S. frugiperda insect cells.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: CaM Kinase II

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      Overview

      Replacement Information
      Description
      OverviewRecombinant, rat Ca2+/calmodulin kinase II α-subunit consisting of amino acids 1-325 (corresponding to monomeric α-subunit) expressed in S. frugiperda insect cells. Reportedly involved in the modulation of neurite outgrowth by cAMP. Lower levels of CaM kinase II α-subunit predispose neurons to ischemic damage.
      Catalogue Number208706
      Brand Family Calbiochem®
      SynonymsCaM Kinase II
      References
      ReferencesTashima, K., et al. 1996. J. Neurochem. 66, 57.
      Waxham, M.N., et al. 1996. J. Cereb. Flow Metab. 16, 1.
      Hanson, P.I., and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.
      Product Information
      Activity≥250,000 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1 pmol phosphate to Autocamtide-2 (Cat. No. 189475), KKALRRQETVDAL, per min at 30°C, pH 7.5.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, 0.02% Tween® 20 Detergent, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Physicochemical Information
      ContaminantsPhosphatases, proteases: none detected
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      208706 0

      Documentation

      Ca2+/Calmodulin Kinase II, α-Subunit, Rat, Recombinant, Spodoptera frugiperda SDS

      Title

      Safety Data Sheet (SDS) 

      Ca2+/Calmodulin Kinase II, α-Subunit, Rat, Recombinant, Spodoptera frugiperda Certificates of Analysis

      TitleLot Number
      208706

      References

      Reference overview
      Tashima, K., et al. 1996. J. Neurochem. 66, 57.
      Waxham, M.N., et al. 1996. J. Cereb. Flow Metab. 16, 1.
      Hanson, P.I., and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-May-2008 JSW
      SynonymsCaM Kinase II
      DescriptionRecombinant, rat Ca2+/calmodulin kinase II α-subunit consisting of amino acids 1-325 (corresponding to monomeric α-subunit) expressed in S. frugiperda insect cells. CaM kinase II is a serine/threonine kinase that phosphorylates a wide variety of intracellular proteins. Reportedly involved in the modulation of neurite growth by cAMP. Lower levels of CaM kinase II a-subunit predispose neurons to ischemic damage.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, 0.02% Tween® 20 Detergent, pH 7.5.
      Recommended reaction conditions1. Dilute CaM kinase II into 20 mM Tris-HCl, 10 mM MgCl2, 0.5 mM DTT, and 0.1 mM EDTA, pH 7.5.2. 2. Add 2.4 μM calmodulin, 2 mM CaCl2, 100 µM ATP, and [32P]-γATP at 100 µCi/µmole 3. Incubate at 30°C.
      Purity≥90% by SDS-PAGE
      ContaminantsPhosphatases, proteases: none detected
      Activity≥250,000 units/ml
      Unit definitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1 pmol phosphate to Autocamtide-2 (Cat. No. 189475), KKALRRQETVDAL, per min at 30°C, pH 7.5.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesTashima, K., et al. 1996. J. Neurochem. 66, 57.
      Waxham, M.N., et al. 1996. J. Cereb. Flow Metab. 16, 1.
      Hanson, P.I., and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.