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400020 Caspase-1 Substrate III, Fluorogenic

Caspase-1 Substrate III, Fluorogenic MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: IL-1β Converting Enzyme (ICE) Substrate III, Ac-YVAD-AMC

400020
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.

      Please refer to our complete listing of caspase substrates for possible alternatives. Please read the alternative product documentation carefully and contact technical service if you need additional information.





      Fluorogenic caspase-1 substrate (Km = 8 µM for human recombinant caspase-1). Also a substrate for caspase-4.
      Catalogue Number400020
      Brand Family Calbiochem®
      SynonymsIL-1β Converting Enzyme (ICE) Substrate III, Ac-YVAD-AMC
      References
      ReferencesThornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
      Walker, N.P.C., et al. 1994. Cell 78, 343.
      Thornberry, N.A., et al. 1992. Nature 356, 768.
      Product Information
      FormWhite solid
      Hill FormulaC₃₃H₃₉N₅O₁₀
      Chemical formulaC₃₃H₃₉N₅O₁₀
      Applications
      Biological Information
      Purity≥90% by HPLC
      Physicochemical Information
      Emission max.
      Excitation max.
      Peptide SequenceAc-Tyr-Val-Ala-Asp-AMC
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Protect from Moisture Protect from moisture
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. DMSO stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      400020 0

      Documentation

      Caspase-1 Substrate III, Fluorogenic Certificates of Analysis

      TitleLot Number
      400020

      References

      Reference overview
      Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
      Walker, N.P.C., et al. 1994. Cell 78, 343.
      Thornberry, N.A., et al. 1992. Nature 356, 768.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision14-July-2008 RFH
      SynonymsIL-1β Converting Enzyme (ICE) Substrate III, Ac-YVAD-AMC
      DescriptionInterleukin-1β converting enzyme (ICE) is a cysteine protease that cleaves the IL-1β precursor to mature IL-1β. The Km and kcat for the hydrolysis of this fluorogenic substrate by rhICE were shown to be 8.5 µM and 0.16 s-1, respectively. This substrate can be used in developing screening assays for ICE inhibitors. Cleavage of the substrate can be monitored using an excitation wavelength of ~365-380 nm and an emission wavelength of ~430-460 nm.
      FormWhite solid
      Chemical formulaC₃₃H₃₉N₅O₁₀
      Peptide SequenceAc-Tyr-Val-Ala-Asp-AMC
      Purity≥90% by HPLC
      SolubilityAcetic acid or DMSO
      Storage Protect from moisture
      Protect from light
      +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. DMSO stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesThornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
      Walker, N.P.C., et al. 1994. Cell 78, 343.
      Thornberry, N.A., et al. 1992. Nature 356, 768.