219366 Cathepsin B, Bovine Spleen

219366
  
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      Overview

      Replacement Information

      Key Spec Table

      Description
      OverviewNative cathepsin B from bovine spleen, purified by affinity chromatography. Cathepsin B, one of the most investigated lysosomal cysteine proteinases, is widely distributed in a variety of tissues. The enzyme exists in a two-polypeptide chain form consisting of an H-chain of 22 - 23 kDa and an L-chain of 5 kDa. Cathepsin B is shown to facilitate tumor invasion by dissolving extracellular barriers.

      This product has been discontinued.





      Catalogue Number219366
      Brand Family Calbiochem®
      References
      ReferencesKatunuma, N., et al. 1998. Adv. Enzyme Regul. 38, 235.
      Aisa, M.C., et al. 1996. Biochim. Biophys. Acta 1290, 29.
      Everts, V., et al. 1996. Histochem. J. 28, 229.
      Product Information
      CAS number9047-22-7
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Z-RR-<i>p</i>NA per min at 37°C, pH 6.0.
      EC number3.4.22.1
      FormLiquid
      FormulationIn 50 mM sodium acetate buffer, 1 mM EDTA, pH 5.0.
      Applications
      Biological Information
      Puritysingle band by non-reducing SDS-PAGE
      Specific Activity≥10 units/mg protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Cathepsin B, Bovine Spleen SDS

      Title

      Safety Data Sheet (SDS) 

      Cathepsin B, Bovine Spleen Certificates of Analysis

      TitleLot Number
      219366

      References

      Reference overview
      Katunuma, N., et al. 1998. Adv. Enzyme Regul. 38, 235.
      Aisa, M.C., et al. 1996. Biochim. Biophys. Acta 1290, 29.
      Everts, V., et al. 1996. Histochem. J. 28, 229.

      Brochure

      Title
      Cathepsins and Related Products Technical Bulletin
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision18-May-2010 JSW
      DescriptionNative cathepsin B from bovine spleen, purified by affinity chromatography. Cathepsin B is the most investigated lysosomal cysteine proteinase distributed in a wide variety of tissues. The enzyme exists in a two-polypeptide chain form consisting of an H-chain of 22-23 kDa and an L-chain of 5 kDa.
      FormLiquid
      FormulationIn 50 mM sodium acetate buffer, 1 mM EDTA, pH 5.0.
      CAS number9047-22-7
      EC number3.4.22.1
      Puritysingle band by non-reducing SDS-PAGE
      Specific activity≥10 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Z-RR-pNA per min at 37°C, pH 6.0.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesKatunuma, N., et al. 1998. Adv. Enzyme Regul. 38, 235.
      Aisa, M.C., et al. 1996. Biochim. Biophys. Acta 1290, 29.
      Everts, V., et al. 1996. Histochem. J. 28, 229.