219463 Cathepsin E, His•Tag®, Mouse, Recombinant, NSO Cells

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
219463
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Recombinant, mouse cathepsin E fused at the C-terminus to a His•Tag® sequence and expressed NSO cells. Cathepsin E is an intracellular aspartic protease of the pepsin family, which has been reported to be involved in the cleavage of the Swedish mutant of amyloid precursor protein. Due to glycosylation, the apparent molecular mass of the dimer is ~100 kDa under non-reducing conditions. Note: Must be activated before use.
      Catalogue Number219463
      Brand Family Calbiochem®
      References
      ReferencesGruninger-Leitch, F., et al. 2000. Nat. Biotechnol. 18, 66.
      Tatnell, P.J., et al. 1997. FEBS Lett. 408, 62.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1 nmol MCA-PLGL(3-DPA-diaminopropionyl)-AR-NH₂ at 25°C, pH 3.5.
      EC number3.4.23.34
      FormLiquid
      FormulationIn 75 mM NaCl, 12.5 mM MES, 50% glycerol, pH 6.5, sterile-filtered.
      Applications
      Biological Information
      Purity≥95 by SDS-PAGE
      Specific Activity≥8 units/μg
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      ContaminantsEndotoxins: ≤1.0 EU/µg enzyme
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Cathepsin E, His•Tag®, Mouse, Recombinant, NSO Cells Certificates of Analysis

      TitleLot Number
      219463

      References

      Reference overview
      Gruninger-Leitch, F., et al. 2000. Nat. Biotechnol. 18, 66.
      Tatnell, P.J., et al. 1997. FEBS Lett. 408, 62.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-June-2008 RFH
      DescriptionRecombinant, mouse cathepsin E fused at the C-terminus to a His•Tag® sequence and expressed NSO cells. Cathepsin E is an intracellular aspartic protease of the pepsin family that has been reported to be involved in the cleavage of the Swedish mutant of amyloid precursor protein (APP). Due to glycosylation the apparent molecular weight of the dimer is ~100 kDa under non-reducing conditions. Requires activation prior to use.
      FormLiquid
      FormulationIn 75 mM NaCl, 12.5 mM MES, 50% glycerol, pH 6.5, sterile-filtered.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditionsActivation Protocol Pre-incubate Cathepsin E for 15 min. at room temperature, at a concentration of 1 µg/ml in 50 mM sodium acetate buffer, pH 3.5, containing 100 mM NaCl. Activity Optimal activity is achieved in 50 mM sodium acetate buffer, pH 3.5, containing 100 mM NaCl, room temperature.
      EC number3.4.23.34
      Purity≥95 by SDS-PAGE
      ContaminantsEndotoxins: ≤1.0 EU/µg enzyme
      Specific activity≥8 units/μg
      Unit definitionOne unit is defined as the amount of enzyme that will cleave 1 nmol MCA-PLGL(3-DPA-diaminopropionyl)-AR-NH₂ at 25°C, pH 3.5.
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesGruninger-Leitch, F., et al. 2000. Nat. Biotechnol. 18, 66.
      Tatnell, P.J., et al. 1997. FEBS Lett. 408, 62.