CYT306 ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA

CYT306
1 kit  
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      Overview

      Replacement Information

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      BDNFH, RELISAChromogenic
      Description
      Catalogue NumberCYT306
      Brand Family Chemicon®
      Trade Name
      • ChemiKine
      • Chemicon
      DescriptionChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA
      OverviewChemiKine™ Brain Derived Neurotrophic Factor (BDNF) kit is a sandwich enzyme immunoassay (EIA), which measures BDNF. The kit will measure BDNF from human and rat.

      Test Principle:

      With the ChemiKine BDNF assay system, rabbit polyclonal antibodies generated against human BDNF are coated onto a microplate and are used to capture BDNF from a sample. BDNF specific, biotin conjugated, mouse monoclonal antibodies detect the captured BDNF. After addition of streptavidin-enzyme, substrate and stop solution the amount of BDNF is determined. The standard curve demonstrates a direct relationship between Optical Density (OD) and BDNF concentration: i.e., the higher the OD the higher the BDNF concentration in the sample.

      Application:

      ChemiKine BDNF kit is designed to measure the amount of BDNF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from human and rat. There are enough reagents included in this kit for two 96-well immuno-assay plates. Running duplicate wells for samples and standards is recommended.

      For Research Use Only. Not for use in diagnostic procedures.
      Alternate Names
      • BDNF
      Materials Required but Not Delivered1. Multi-channel or repeating pipettes

      2. Plate shaker (optional)

      3. Pipettors & tips capable of accurately measuring 10-1000 mL

      4. Graduated serological pipettes

      5. 96-well microplate Reader with 450 nm filter

      6. Graph paper for manual plotting of data

      7. Polystyrene test tubes for standard and sample dilutions

      8. Mechanical vortex

      9. One 1 or 2 liter container
      References
      Product Information
      Components
      • ChemiKine BDNF ELISA Plate: (Part No. 60238) Two Pre-coated with Rabbit anti-Human BDNF Polyclonal Antibody 96-Well Immunoplates sealed in foil pouches.
      • Wash Buffer Concentrate: (Part No. 60245) One 100 mL (10X) bottle of Concentrate.
      • Standard/Sample Diluent: (Part No. 60240) One 60 mL bottle (Ready to Use).
      • BDNF Standard (Recombinant Human): (Part No. 60237) Two vials (Lyophilized).
      • Biotinylated Mouse anti-Human BDNF Monoclonal Antibody: (Part No. 60583) One 25 μL vial.
      • Streptavidin-Enzyme conjugate: (Part No. 60582) One 50 mL vial of HRP conjugated Streptavidin.
      • TMB/E Solution: (Part No. 60096) Two 10 mL bottles of a Ready to Use solution of 3,3',5,5'-tetramethylbenzidine in a proprietary buffer with enhancer.
      • Stop Solution (Part No. 60260): One 22 mL bottle of an HCl solution.
      Detection methodChromogenic
      Quality LevelMQ100
      Applications
      ApplicationThis ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA is used to measure & quantify BDNF levels in Neuroscience research.
      Key Applications
      • ELISA
      Biological Information
      Species Reactivity
      • Human
      • Rat
      Analytes Available
      • BDNF
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene, but the full-length nature of only some could be determined.
      Gene Symbol
      • BDNF
      • Abrineurin
      • neurotrophin
      • MGC34632
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P23560 # Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
      SIZE: 247 amino acids; 27818 Da
      SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB.
      SUBCELLULAR LOCATION: Secreted.
      PTM: The propeptide is N-glycosylated and glycosulfated. & Converted into mature BDNF by plasmin (PLG) (By similarity).
      DISEASE: SwissProt: P23560 # Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases.
      SIMILARITY: SwissProt: P23560 ## Belongs to the NGF-beta family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • For research use only. Not for use in diagnostic procedures.
      Storage and Shipping Information
      Storage ConditionsPrecautions:

      · Wash Buffer and Sample Buffer contain thimerosal. Thimerosal is highly toxic by inhalation, contact with skin or if swallowed. Thimerosal is a possible mutagen and should be handled accordingly.

      · The instructions provided have been designed to optimize the kit's performance. Deviation from the instructions may result in suboptimal performance of the kit and the failure to produce accurate data.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewSpeciesPub Med ID
      Expression and localization of brain-derived neurotrophic factor (BDNF) mRNA and protein in human submandibular gland.
      Juri Saruta,Kazuhiro Fujino,Masahiro To,Keiichi Tsukinoki
      Acta histochemica et cytochemica  45  2012

      Show Abstract
      23012486 23012486
      Various levels of plasma brain-derived neurotrophic factor in patients with tinnitus.
      Fumiyuki Goto,Juri Saruta,Sho Kanzaki,Masahiro To,Tomoko Tsutsumi,Keiichi Tsukinoki,Kaoru Ogawa
      Neuroscience letters  510  2012

      Show Abstract
      22281446 22281446
      Decreased levels of brain-derived neurotrophic factor in the remitted state of unipolar depressive disorder.
      B J Hasselbalch,U Knorr,B Bennike,S G Hasselbalch,M H Greisen Søndergaard,L Vedel Kessing
      Acta psychiatrica Scandinavica  126  2012

      Show Abstract
      22292883 22292883
      BDNF as an effect modifier for gender effects on pain thresholds in healthy subjects.
      Luciana Cadore Stefani,Iraci Lucena da Silva Torres,Izabel Cristina Custodio de Souza,Joanna Ripoll Rozisky,Felipe Fregni,Wolnei Caumo
      Neuroscience letters  514  2012

      Show Abstract
      22395087 22395087
      Propofol interacts with stimulus intensities of electroconvulsive shock to regulate behavior and hippocampal BDNF in a rat model of depression.
      Jie Luo,Su Min,Ke Wei,Junfang Zhang,Yuanyuan Liu
      Psychiatry research  198  2012

      Show Abstract
      22410590 22410590
      Prolonged therapeutic hypothermia does not adversely impact neuroplasticity after global ischemia in rats.
      Gergely Silasi,Ana C Klahr,Mark J Hackett,Angela M Auriat,Helen Nichol,Frederick Colbourne
      Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism  32  2012

      Show Abstract
      22434072 22434072
      Serum MIP-1 alpha level: a biomarker for the follow-up of lentiviral therapy in mucopolysaccharidosis IIIB mice.
      Paola Di Natale,Carmela Di Domenico,Daniele Di Napoli
      Journal of inherited metabolic disease  33  2010

      Show Abstract
      20162365 20162365
      Increase in brain-derived neurotrophic factor in first episode psychotic patients after treatment with atypical antipsychotics.
      González-Pintoa A, Mosquera F, Palomino A, Alberich S, Gutiérrez A, Haidar K, Vega P, Barbeito S, Ortiz A, Matute C
      Int Clin Psychopharmacol  25  241-5.  2010

      Show Abstract
      20568658 20568658
      IFATS collection: The conditioned media of adipose stromal cells protect against hypoxia-ischemia-induced brain damage in neonatal rats.
      Xing Wei, Zhimei Du, Liming Zhao, Dongni Feng, Gang Wei, Yongzheng He, Jiangning Tan, Wei-Hui Lee, Harald Hampel, Richard Dodel, Brian H Johnstone, Keith L March, Martin R Farlow, Yansheng Du
      Stem cells (Dayton, Ohio)  27  478-88  2009

      Show Abstract
      19023032 19023032
      The differential regulation of BDNF and TrkB levels in juvenile rats after four days of escitalopram and desipramine treatment.
      Megan E Kozisek, David Middlemas, David B Bylund
      Neuropharmacology  54  251-7  2008

      Show Abstract
      Rat18048068 18048068

      Brochure

      Title
      Custom Immunoassay Menu of Services

      Data Sheet

      Title
      Full Service Custom-built Assays

      Instructions for Use

      Title
      ChemiKine¿ Brain Derived Neurotrophic Factor (BDNF) Sandwich ELISA Kit