220487 Chk2, His•Tag®, Human, Recombinant, S. frugiperda

220487
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Recombinant, human checkpoint homologue kinase 2 (Chk2) fused to a His•Tag® sequence at the N-terminus and expressed in S. frugiperda insect cells. Chk2 is involved in sensing DNA damage in eukaryotic cells. It plays an important role in the control of the cell cycle by blocking replication.
      Catalogue Number220487
      Brand Family Calbiochem®
      SynonymsCHK2 checkpoint homolog, CDS1, RAD53, βA44G7, CHEK2
      Application Data
      Increasing amounts of Chk2, His•Tag®, Active, Human, Recombinant, S. frugiperda were assayed for activity as outlined above.
      References
      ReferencesBartek, J., et al. 2001. Nat. Rev. Mol. Cell Biol. 12, 877.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme required to transfer 1 picomol phosphate to the synthetic peptide substrate KKKVSRSGLYRSPSMPENLNRPR per min at 30°C.
      FormLiquid
      FormulationIn 270 mM sucrose, 150 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 0.1 mM EGTA, pH 7.5.
      Applications
      Biological Information
      Purity≥ 95% by SDS PAGE
      Specific Activity≥ 25,000 units/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Chk2, His•Tag®, Human, Recombinant, S. frugiperda Certificates of Analysis

      TitleLot Number
      220487

      References

      Reference overview
      Bartek, J., et al. 2001. Nat. Rev. Mol. Cell Biol. 12, 877.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-August-2007 JSW
      SynonymsCHK2 checkpoint homolog, CDS1, RAD53, βA44G7, CHEK2
      Application Data
      Increasing amounts of Chk2, His•Tag®, Active, Human, Recombinant, S. frugiperda were assayed for activity as outlined above.
      DescriptionRecombinant, human checkpoint homologue kinase 2 (Chk2) fused to a His•Tag® sequence at the N-terminus and expressed in S. frugiperda insect cells. Chk2 is involved in sensing DNA damage in eukaryotic cells. It plays an important role in the control of the cell cycle by blocking replication.
      FormLiquid
      FormulationIn 270 mM sucrose, 150 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 0.1 mM EGTA, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      Kinase Activity Assay Materials Required • Reaction Buffer: 40 mM MOPS, 2 mM EDTA, pH 7.5 • Substrate: 200 µM CHK peptide substrate, KKKVSRSGLYRSPSMPENLNRPR • Kinase Dilution Buffer: 20 mM MOPS, 10 mM DTT, 1 mM EDTA, 1 mg/ml BSA, 5% glycerol, pH 7.5 • Chk2 Kinase: Chk2, His•Tag®, Active, Human, Recombinant, S. frugiperda diluted to 50-100 ng/3 µl in Kinase Dilution Buffer • Magnesium/ATP Cocktail: 37.5 mM MgCl2, 250 µM ATP • Diluted [γ-32P]ATP: 197 µl Magnesium/ATP Cocktail with 3 µl (30 µCi) [γ-32P]ATP (3000 Ci/mmol) • 0.75% Phosphoric Acid • Acetone Protocol 1. Mix the following components in the specified order in a microcentrifuge tube on ice: • 4 µl Reaction Buffer • 3 µl Substrate • 3 µl Chk2 Kinase (50-100 ng/assay) • 10 µl Diluted [γ-32P]ATP 2. Incubate at 30°C for 10 min. 3. Stop the reaction by placing the tubes on ice. 4. Remove 10 µl of the reaction and spot on P81 paper; allow to bind for 30 s. 5. Immerse the paper in 0.75% Phosphoric Acid and shake gently on a rotator. Discard the Phosphoric Acid and repeat for a total of 3 washes. 6. Wash the paper with acetone. 7. Dry using infrared light. 8. Read the results using a scintillation counter.
      Purity≥ 95% by SDS PAGE
      Specific activity≥ 25,000 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme required to transfer 1 picomol phosphate to the synthetic peptide substrate KKKVSRSGLYRSPSMPENLNRPR per min at 30°C.
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesBartek, J., et al. 2001. Nat. Rev. Mol. Cell Biol. 12, 877.