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17-10454 CpGenome Direct Prep-96 Bisulfite Modification Kit (192 Reactions)

96-well plate-based bisulfite conversion of DNA directly from a variety of starting materials, including cultured cells, blood, fresh tissue & fixed tissue samples. Study methylation directly without genomic DNA isolation.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
17-10454
192 reactions (2x96-well plates)  
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      Overview

      Replacement Information
      Description
      Catalogue Number17-10454
      Trade Name
      • CpGenome
      DescriptionCpGenome Direct Prep-96 Bisulfite Modification Kit (192 Reactions)
      OverviewUnlike other bisulfite modifications approaches that start with isolated genomic DNA, the CpGenome Direct Prep-96 Bisulfite Modification Kit allows simple and reliable 96-well plate-based bisulfite conversion of DNA directly from a variety of starting materials, including cultured cells, blood, fresh tissue and fixed tissue samples.

      Key Features
      • 96-well plate-based bisulfite conversion directly from cells, tissues, blood and FFPE samples without DNA purification
      • Sensitive bisulfite-conversion from as few as 10 cells or as low as 50 pg of input DNA
      • Fast and simple, streamlined protocol for one-step bisulfite conversion
      • On-plate desulfonation allows recovery of DNA without additional precipitation steps for more consistent results
      • Suitable for downstream analysis by methlylation specific PCR, restriction digestion, sequencing, microarray hybridization, etc.
      Materials Required but Not DeliveredReagents
      • Nuclease-free water
      • 96-100% Molecular Biology Grade Ethanol
      • Xylene (For FFPE Samples)

      Equipment and Supplies
      • Thermal cycler, heat block, or water bath
      • Vortex or Eppendorf Thermomixer®
      • Microcentrifuge (capable of 12,000 x g)
      • Microcentrifuge tubes, 1.5-2.0 mL
      • Pipettes and pipette tips (aerosol barrier tips are suggested to minimize potential cross contamination).
      • Analytical scale or balance (For weighing tissue samples)
      • Untreated glass slides and slide washing chambers (For FFPE Samples)
      Background InformationMethylation of cytosines located 5' to guanosine is known to have a profound effect on the expression of many eukaryotic genes. In normal cells methylation occurs predominantly in CG-poor regions, while CG-rich areas, called CpG-islands remain unmethylated. The exceptions are the extensive methylation of CpG islands associated with transcriptional inactivation of regulatory regions of imprinted genes and genes on the inactive X-chromosome of females. Aberrant methylation of normally unmethylated CpG islands has been documented as a relatively frequent event in immortalized and transformed cells and has been associated with transcriptional inactivation of defined tumor suppressor genes in human cancers. Hundreds of CpG islands are now known to exhibit the characteristic of hypermethylation in tumors resulting in identification of candidate genes that can be interrogated to determine extent of tumor specific transformation.
      References
      Product Information
      Components
      • 2X Extraction Buffer
      • Modification Reagent
      • Conversion Buffer
      • Resuspension Buffer
      • Equilibration Buffer
      • Binding Buffer
      • Wash Buffer I
      • Wash Buffer II
      • Elution Buffer
      • Proteinase K
      • Proteinase K Storage Buffer
      • Conversion Plates/Cover films
      • Desalting Plates
      • Collection Plates
      • Elution Plates
      Quality LevelMQ100
      Applications
      Application96-well plate-based bisulfite conversion of DNA directly from a variety of starting materials, including cultured cells, blood, fresh tissue & fixed tissue samples. Study methylation directly without genomic DNA isolation.
      Application NotesFor MSP primer design, please use the MethPrime software package. Click here
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size192 reactions (2x96-well plates)
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      17-10454 04053252947209