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PF122 CrmA, Recombinant

CrmA, Recombinant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Primary Target: human caspase-1 and Granzyme B
PF122
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      CrmA (cowpox viral serpin cytokine response modifier A) is purified from E. coli transformed with a construct containing the full-length coding region of the CrmA gene and 7 additional amino acids that do not affect the activity. CrmA is a natural inhibitor of human caspase-1 and granzyme B, enzymes that are involved in apoptosis.
      Catalogue NumberPF122
      Brand Family Calbiochem®
      References
      ReferencesSnipas, S.J., et al. 2001. Biochem. J. 357, 575.
      Quan, L.T., et al. 1995. J. Biol. Chem. 270, 10377.
      Product Information
      FormLiquid
      FormulationIn 100 mM NaCI, 20 mM HEPES, pH 7.4.
      Applications
      Biological Information
      Primary Targethuman caspase-1 and Granzyme B
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PF122 0

      Documentation

      CrmA, Recombinant Certificates of Analysis

      TitleLot Number
      PF122

      References

      Reference overview
      Snipas, S.J., et al. 2001. Biochem. J. 357, 575.
      Quan, L.T., et al. 1995. J. Biol. Chem. 270, 10377.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-September-2008 RFH
      DescriptionFull-length recombinant CrmA expressed in E. coli with 7 non-native amino acids (without affecting activity). Cowpox viral serpin cytokine response modifier A (CrmA) is a proteinase inhibitor that prevents programmed cell death initiated by a variety of stimuli. CrmA is selective in its ability to inhibit caspases, showing high affinity for interleukin-1β-converting enzyme (Caspase-1) and Granzyme B, enzymes that are involved in apoptosis.
      Specieshuman
      FormLiquid
      FormulationIn 100 mM NaCI, 20 mM HEPES, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditionsFor highest inhibitory activity, activate CrmA with 2 mM DTT for 5 min at room temprature. If granzymeB room temperature. If granzymeB thiol ester-based substrate assays are to be preformed, the excess DTT can be blocked by adding 4 mM NaAsO2 to the activated CrmA.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesSnipas, S.J., et al. 2001. Biochem. J. 357, 575.
      Quan, L.T., et al. 1995. J. Biol. Chem. 270, 10377.