268298 DAPI, Dihydrochloride - CAS 28718-90-3 - Calbiochem

268298
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Overview

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Key Spec Table

Empirical FormulaCAS #
C₁₆H₁₅N₅ · 2HCl28718-90-3

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268298-10MG
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      Plastic ampoule 10 mg
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      Description
      OverviewCell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Also available as a 5 mg/ml mM solution in H2O (Cat. No. 508741).
      Catalogue Number268298
      Brand Family Calbiochem®
      Synonyms4ʹ,6-Diamidino-2-phenylindole, 2HCl
      References
      ReferencesMathieu, C., et al. 1995. Exp. Cell Res. 216, 13.
      Cubria, C., et al. 1994. Comp. Biochem. Physiol. C. 105, 251.
      Hotz, M.A., et al. 1994. Cytometry 15, 237.
      Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods 26, 249.
      Miller, T., et al. 1993. Biotechniques 15, 1042.
      Gray, J.W., et al. 1982. Science 238, 323.
      Hessling, J.J., et al. 1980. J. Immunol. Methods 38, 315.
      Russels, W.C., et al. 1975. Nature 253, 461.
      Product Information
      CAS number28718-90-3
      ATP CompetitiveN
      FormYellow solid
      Hill FormulaC₁₆H₁₅N₅ · 2HCl
      Chemical formulaC₁₆H₁₅N₅ · 2HCl
      Hygroscopic Hygroscopic
      ReversibleY
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetBinds preferentially to DNA rich in adenine and thymine
      Purity≥90% by HPLC
      Physicochemical Information
      Cell permeableN
      Emission max.
      Excitation max.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSNL5995050
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C or for up to 6 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      DAPI, Dihydrochloride - CAS 28718-90-3 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      DAPI, Dihydrochloride - CAS 28718-90-3 - Calbiochem Certificates of Analysis

      TitleLot Number
      268298

      References

      Reference overview
      Mathieu, C., et al. 1995. Exp. Cell Res. 216, 13.
      Cubria, C., et al. 1994. Comp. Biochem. Physiol. C. 105, 251.
      Hotz, M.A., et al. 1994. Cytometry 15, 237.
      Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods 26, 249.
      Miller, T., et al. 1993. Biotechniques 15, 1042.
      Gray, J.W., et al. 1982. Science 238, 323.
      Hessling, J.J., et al. 1980. J. Immunol. Methods 38, 315.
      Russels, W.C., et al. 1975. Nature 253, 461.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-June-2008 RFH
      Synonyms4ʹ,6-Diamidino-2-phenylindole, 2HCl
      DescriptionFluorescent dye specific for DNA. Binds preferentially to DNA rich in adenine and thymidine (excitation max. = 359 nm, emission max. = 461 nm). Useful for microscopic analysis of nuclei and nuclear DNA in apoptotic cells. Can also be used to detect mycoplasma. Reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.
      FormYellow solid
      CAS number28718-90-3
      RTECSNL5995050
      Chemical formulaC₁₆H₁₅N₅ · 2HCl
      Structure formulaStructure formula
      Purity≥90% by HPLC
      SolubilityEthanol, H₂O (5 mg/ml), or Methanol
      Storage -20°C
      Hygroscopic
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C or for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesMathieu, C., et al. 1995. Exp. Cell Res. 216, 13.
      Cubria, C., et al. 1994. Comp. Biochem. Physiol. C. 105, 251.
      Hotz, M.A., et al. 1994. Cytometry 15, 237.
      Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods 26, 249.
      Miller, T., et al. 1993. Biotechniques 15, 1042.
      Gray, J.W., et al. 1982. Science 238, 323.
      Hessling, J.J., et al. 1980. J. Immunol. Methods 38, 315.
      Russels, W.C., et al. 1975. Nature 253, 461.