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69734 λDE3 Lysogenization Kit - Novagen

69734
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      Description
      OverviewThe λDE3 Lysogenization Kit is designed for site-specific integration of λDE3 prophage into an E. coli host cell chromosome, such that the lysogenized host can be used to express target genes cloned in pET vectors. λDE3 is a recombinant phage carrying the cloned gene for T7 RNA polymerase under lacUV5 control. Lysogens are prepared by co-infection with the three provided phages, and can be verified by plating the T7 Tester Phage (a T7 RNA polymerase deletion mutant). This phage is unable to make a plaque on cells that lack T7 RNA polymerase, but makes normal plaques on λDE3 lysogens in the presence of IPTG.The T7 RNA Polymerase Monoclonal Antibody can be used to verify the presence of T7 RNA polymerase in the host strain by Western blot analysis.

      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number69734
      Brand Family Novagen®
      References
      Product Information
      200 µlλDE3 Phage Lysate
      200 µlHelper Phage Lysate
      200 µlSelection Phage Lysate
      50 µlT7 Tester Phage Lysate
      200 µlHMS174(DE3) glycerol stock
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      λDE3 Lysogenization Kit - Novagen SDS

      Title

      Safety Data Sheet (SDS) 

      λDE3 Lysogenization Kit - Novagen Certificates of Analysis

      TitleLot Number
      69734

      Citations

      Title
    • Daniela A. Geisler, et al. (2007) Ca2+-binding and Ca2+-independent respiratory NADH and NADPH dehydrogenases of Arabidopsis thaliana. Journal of Biological Chemistry 282, 28455-28464.
    • Jan-Willem de Kraker, et al. (2007) Two Arabidopsis genes (IPMS1 and IPMS2) encode isopropylmalate synthase, the branchpoint step in the biosynthesis of leucine. Plant Physiology 143, 970-986.
    • Susanne Textor, et al. (2007) MAM3 catalyzes the formation of all Aliphatic glucosinolate chain lengths in Arabidopsis. Plant Physiology 144, 60-71.
    • Samridhi C. Goswami, et al. (2006) Molecular and functional interactions between Escherichia coli nucleoside diphosphate kinase and the uracil-DNA glycosylase ung. Journal of Biological Chemistry 281, 32131-32139.
    • Sandra Angelini, Sandra Deitermann and Hans-Georg Koch. (2005) FtsY, the bacterial signal-recognition particle receptor, interacts functionally and physically with the SecYEG translocon. European Molecular Biology Organization Reports 6, 476-481.
    • Andrew Karla, et al. (2005) The identification of residues that control signal peptidase cleavage fidelity and substrate specificity. Journal of Biological Chemistry 280, 6731-6741.
    • Dong-Hyun Lee, et al. (2005) Repair of methylation damage in DNA and RNA by mammalian AlkB homologues. Journal of Biological Chemistry 280, 39448-39459.
    • Paul R. Wheeler, et al. (2005) Functional demonstration of reverse transsulfuration in the Mycobacterium tuberculosis complex reveals that methionine is the preferred sulfur source for pathogenic Mycobacteria. Journal of Biological Chemistry 280, 8069-8078.
    • Junhua Zhao and Alan M. Lambowitz. (2005) A bacterial group II intron-encoded reverse transcriptase localizes to cellular poles. Proceedings of the National Academy of Sciences (USA) 102, 16133-16140.
    • Dhammika Gunasekera and Robert G. Kemp. (1999) Cloning, sequencing, expression, and purification of the C isozyme of mouse phosphofructokinase. Protein Expression and Purification 16, 448-453.
    • G. Chang, et al. (1998) Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel. Science 282, 2220-2226.
    • D.J. Hosfield, et al. (1998) Structure of the DNA repair and replication endonuclease and exonuclease FEN-1: coupling DNA and PCNA binding to FEN-1 activity. Cell 95, 135-146.
    • R. Morgan, J.-P. Xiao and S.-Y. Xu. (1998) Characterization of an extremely thermostable restriction enzyme, PspG I from a Pyrococcus strain and cloning of the PspG I restriction-modification system in Escherichia coli. Applied and Enviornmental Microbiology 64, 3669-3673.
    • H. Chen, G. Ferbeyre and R. Cedergren. (1997) Efficient hammerhead ribozyme and antisense RNA targeting in a slow ribosome Escherichia coli mutant. Nature Biotechnology 15, 432-435.
    • Kwei-Lan Tsao and David S. Waugh. (1997) Balancing the production of two recombinant proteins in Escherichia coli by manipulating plasmid copy number: high-level expression of heterodimeric Ras farnesyltransferase. Protein Expression and Purification 11, 233-240.
    • G.-L. Xu and T.H. Bestor. (1997) Cytosine methylation targetted to pre-determined sequences. Nature Genetics 17, 376-378.
    • User Protocols

      Title
      TB031 lDE3 Lysogenization Kit

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