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ASK23 Downstream Effector Caspase Sampler Kit

ASK23
  
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      Overview

      Replacement Information
      Description
      OverviewContains 20 µg of each of the following antibodies: Anti-Caspase-3 (Cat. Nos. AM34 and AM39), Anti-Caspase-6 (Cat. Nos. PC365 and PC416), and Anti-Caspase-7 (Cat. Nos. AM45 and PC334). Please see individual product descriptions for further information.
      Catalogue NumberASK23
      Brand Family Calbiochem®
      References
      ReferencesCryns, V., and Yuan, J., 1998. Genes Dev. 12, 1551.
      Wolf, B., and Green, D., 1999. J. Biol. Chem. 274, 20049.
      Ashkenazi, A., and Dixit, V., 1998. Science 281, 1305.
      Cohen, G., 1997. Biochem. J. 326, 1.
      Nicholson, S., and Thornberry, N., 1997. Trends Biochem. Sci. 22, 299.
      Product Information
      ProductIsotypeCloneSpecies ReactivityApplicationCat. No.
      Caspase-3 (Ab-2) Monoclonal AntibodyIgG₁10C1.C9HumanWB, not FS, IF, IP or PS AM34
      Caspase-3 (Ab-3) Monoclonal AntibodyIgG2aAM1.4.1-1B HumanWB, not FS, IP or PS AM39
      Caspase-6 (Ab-1) Polyclonal AntibodyIgGHuman, MouseWB PC365
      Caspase-6 (Ab-2) Polyclonal AntibodyIgGHuman, Mouse, RatWB PC416
      Caspase-7 (Ab-1) Polyclonal AntibodyIgGHumanWB PC334
      Caspase-7 (Ab-2) Monoclonal AntibodyIgG₁I-10Human, not Mouse or RatWB AM45
      FormLiquid
      Positive controlHeLa or MCF7 cells induced for apoptosis
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (see characteristics below)
      Frozen Sections (see characteristics below)
      Immunofluorescence (see characteristics below)
      Immunoprecipitation (see characteristics below)
      Paraffin Sections (see characteristics below)
      Application CommentsSuggested starting concentrations are provided on individual data sheets. Both primary and secondary antibodies should be titrated for optimal results in individual systems.
      Biological Information
      IsotypeIgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      References

      Reference overview
      Cryns, V., and Yuan, J., 1998. Genes Dev. 12, 1551.
      Wolf, B., and Green, D., 1999. J. Biol. Chem. 274, 20049.
      Ashkenazi, A., and Dixit, V., 1998. Science 281, 1305.
      Cohen, G., 1997. Biochem. J. 326, 1.
      Nicholson, S., and Thornberry, N., 1997. Trends Biochem. Sci. 22, 299.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-February-2008 RFH
      ApplicationImmunoblotting (see characteristics below)
      Frozen Sections (see characteristics below)
      Immunofluorescence (see characteristics below)
      Immunoprecipitation (see characteristics below)
      Paraffin Sections (see characteristics below)
      DescriptionSet of 6 mouse monoclonal antibodies specific for caspase-3, caspase-6, or caspase-7.
      BackgroundThe caspase family of proteins plays a central role in the apoptotic response. Normally existing in a latent, zymogen form, following receipt of an apoptotic signal they become activated and proceed to cleave and dismantle the structural, regulatory and genetic information in the cell either directly or indirectly. There are currently over a dozen caspase proteins that have been described in multiple species and many of these proteins exist as isoform variants. The spectrum of caspases can be broadly divided into three families based either on structural motifs held in common or on the basis of activity. These families are the CARD containing caspases, the DED containing caspases and the downstream effector caspases. The downstream effector caspases are those responsible for direct cleavage of cellular substrates and most likely responsible for the "death-blow" during apoptosis. This group contains caspases 3, 6, and 7.
      IsotypeIgG
      Positive controlHeLa or MCF7 cells induced for apoptosis
      FormLiquid
      CommentsSuggested starting concentrations are provided on individual data sheets. Both primary and secondary antibodies should be titrated for optimal results in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesCryns, V., and Yuan, J., 1998. Genes Dev. 12, 1551.
      Wolf, B., and Green, D., 1999. J. Biol. Chem. 274, 20049.
      Ashkenazi, A., and Dixit, V., 1998. Science 281, 1305.
      Cohen, G., 1997. Biochem. J. 326, 1.
      Nicholson, S., and Thornberry, N., 1997. Trends Biochem. Sci. 22, 299.