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SCC284 EpH4 Mouse Mammary Epithelial Cell Line

EpH4 mouse mammary epithelial cell line is a versatile system for studying a spectrum of morphological transformation spanning breast cancer metastasis, EMT, and hormonal regulation.

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SCC284
≥1X10⁶ cells/vial   
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCC284
      DescriptionEpH4 Mouse Mammary Epithelial Cell Line
      Alternate Names
      • EPH4
      • EpH-4
      • Eph4
      Background InformationMammary epithelial cells are a model for cellular plasticity, the ability to undergo varied morphological changes. One of the significant aspects of mammary-derived tumors is epithelial-to-mesenchymal transition (EMT), which gives rise to the migratory behavior required for metastasis (1). Cellular plasticity makes mammary epithelial cell models valuable for the study of breast cancer malignancy in addition to hormonal regulation of differentiation and epithelial cell polarization.

      Eph4 is a spontaneously immortalized cell line derived from primary murine mammary epithelial cells (2). EpH4 cells can be induced to synthesize milk proteins, such as caseins, upon the induction with lactogenic hormones such as prolactin, hydrocortisone, and insulin. For normal maintenance Eph4 cells should be cultured at high density (semi-confluency) and passaged before reaching confluency to avoid polarization and selective culture of single cells. Post-confluent Eph4 cells undergo polarization and formation of hemicysts or dome-like structures characterized by unidirectional transport of ions (3).

      Source:
      Eph4 cell line is a spontaneously immortalized clone derived from primary mammary epithelial cells of a female BALB/c mouse.

      References:
      1. Breast Cancer Res. 2005; 7(4): 171-179.
      2. EMBO J 1995; 14(6): 1145-55.
      3. J Cell Biol. 1989; 108(3): 1127-38.
      References
      Product Information
      Applications
      ApplicationEpH4 mouse mammary epithelial cell line is a versatile system for studying a spectrum of morphological transformation spanning breast cancer metastasis, EMT, and hormonal regulation.
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Epithelial Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
      • Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are verified to be of mouse origin and negative for inter-species contamination from rat, human, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are negative for mycoplasma contamination.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCC284 04061842104017