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341591 FGF-1, Acidic, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: rhaFGF, Fibroblast Growth Factor, Acidic, Human, Recombinant, E. coli

341591
  
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      Overview

      Replacement Information

      Key Spec Table

      Purity
      ≥97% by SDS-PAGE
      Description
      Overview

      This product has been discontinued.





      Recombinant, human acidic fibroblast growth factor expressed in E. coli. Stimulates the proliferation of primary cultures of myoblasts and osteoblastic cells. Prevents myocardial apoptosis triggered by ischemia reperfusion injury. Exhibits mitogenic activity in vitro for cells or meso- and neuroectodermal origin. Its activity is greatly potentiated by addition of heparin.
      Catalogue Number341591
      Brand Family Calbiochem®
      SynonymsrhaFGF, Fibroblast Growth Factor, Acidic, Human, Recombinant, E. coli
      References
      ReferencesCuevas, P., et al. 1997. Eur. J. Med. Res. 2, 465.
      Junttila, T., et al. 1996. Brain Res. 707, 81.
      Baird, A., et al. 1994. Curr. Opin. Neuro. Biol. 4, 78.
      Gospodarowicz, D. 1991. Ann. N.Y. Acad. Sci. 638, 1.
      Rizzino, A., et al. 1988. Cancer Res. 48, 4266.
      Giminez-Gallego, G., et al. 1986. Biochem. Biophys. Res. Commun. 138, 611.
      Harper, J.W., et al. 1986. Biochemistry 25, 4097.
      Jaye, M., et al. 1986. Science 233, 541.
      Thomas, K.A., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 357.
      Product Information
      CAS number106096-92-8
      FormSolid
      FormulationLyophilized from a sterile-filtered solution of 20 mM Tris, 1 M NaCl, 50 µg BSA/µg FGF acidic, pH 7.0.
      ReconstitutionReconstitute to a concentration of ≥1 μg/ml with sterile PBS containing at least 0.1% HSA or BSA. Reconstituted material can be stored at +4°C for up to 3 months without detectable loss of activity. For longer storage, aliquot and freeze at -70°C. Avoid freeze/thaw cycles of solutions.
      Applications
      Biological Information
      Biological activityED₅₀ = 100-300 pg/ml in the presence of 10 µg/ml heparin as monitored in a mitogenic assay by measuring the acidic FGF-dependent ³H-thymidine incorporation in quiescent NR6R/3T3 fibroblasts
      Purity≥97% by SDS-PAGE
      Physicochemical Information
      Contaminantsendotoxin: ≤ 1 EU/µg FGF
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSLK3641300
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at 4°C or for up to 6 months at -70°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      341591 0

      Documentation

      References

      Reference overview
      Cuevas, P., et al. 1997. Eur. J. Med. Res. 2, 465.
      Junttila, T., et al. 1996. Brain Res. 707, 81.
      Baird, A., et al. 1994. Curr. Opin. Neuro. Biol. 4, 78.
      Gospodarowicz, D. 1991. Ann. N.Y. Acad. Sci. 638, 1.
      Rizzino, A., et al. 1988. Cancer Res. 48, 4266.
      Giminez-Gallego, G., et al. 1986. Biochem. Biophys. Res. Commun. 138, 611.
      Harper, J.W., et al. 1986. Biochemistry 25, 4097.
      Jaye, M., et al. 1986. Science 233, 541.
      Thomas, K.A., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 357.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-May-2008 RFH
      SynonymsrhaFGF, Fibroblast Growth Factor, Acidic, Human, Recombinant, E. coli
      DescriptionRecombinant, human acidic fibroblast growth factor expressed in E. coli. Composed of 140 amino acid residues. Stimulates the proliferation of primary cultures of osteoblastic cells. Found in brain, retina, bone matrix, and in osteosarcoma cells. Exhibits mitogenic activity in vitro for cells of meso- and neuroectodermal origin. Has a role in wound healing, induction of neuron differentiation, and modulation of embryonic development and differentiation. Acts as a chemoattractant and has mitogenic activity in vitro for endothelial cells and angiogenic activity in vivo. Activity is greatly potentiated by the addition of heparin.
      FormSolid
      FormulationLyophilized from a sterile-filtered solution of 20 mM Tris, 1 M NaCl, 50 µg BSA/µg FGF acidic, pH 7.0.
      CAS number106096-92-8
      RTECSLK3641300
      Purity≥97% by SDS-PAGE
      Contaminantsendotoxin: ≤ 1 EU/µg FGF
      Biological activityED₅₀ = 100-300 pg/ml in the presence of 10 µg/ml heparin as monitored in a mitogenic assay by measuring the acidic FGF-dependent ³H-thymidine incorporation in quiescent NR6R/3T3 fibroblasts
      SolubilityReconstitute to ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at 4°C or for up to 6 months at -70°C.
      Toxicity Standard Handling
      Merck USA index14, 4072
      ReferencesCuevas, P., et al. 1997. Eur. J. Med. Res. 2, 465.
      Junttila, T., et al. 1996. Brain Res. 707, 81.
      Baird, A., et al. 1994. Curr. Opin. Neuro. Biol. 4, 78.
      Gospodarowicz, D. 1991. Ann. N.Y. Acad. Sci. 638, 1.
      Rizzino, A., et al. 1988. Cancer Res. 48, 4266.
      Giminez-Gallego, G., et al. 1986. Biochem. Biophys. Res. Commun. 138, 611.
      Harper, J.W., et al. 1986. Biochemistry 25, 4097.
      Jaye, M., et al. 1986. Science 233, 541.
      Thomas, K.A., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 357.

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      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors