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343242 FLUO 3/AM - CAS 121714-22-5 - Calbiochem

343242
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Overview

Replacement Information

Key Spec Table

Empirical FormulaCAS #
C₅₁H₅₀Cl₂N₂O₂₃121714-22-5

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343242-1MG
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      Description
      OverviewMembrane-permeable version of FLUO 3 (Cat. No. 343244). Unlike all other Ca2+ indicators, FLUO 3/AM is non-fluorescent until it is hydrolyzed in the cell by cellular esterases. It is insensitive to Mg2+.
      Catalogue Number343242
      Brand Family Calbiochem®
      Synonyms1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)]-2-(2ʹ-amino-5ʹ-methylphenoxy)ethane-N,N,Nʹ,Nʹ-tetraacetic Acid Pentaacetoxymethyl Ester
      References
      ReferencesCaputo, C., and Bolanos, P. 1994. J. Physiol. (London) 481, 119.
      Schumann, M.A., et al. 1993. J. Biol. Chem. 268, 2134.
      Eberhard, M., and Erne, P. 1989. Biochem. Biophys. Res. Commun. 163, 309.
      Product Information
      CAS number121714-22-5
      ATP CompetitiveN
      FormReddish orange solid
      Hill FormulaC₅₁H₅₀Cl₂N₂O₂₃
      Chemical formulaC₅₁H₅₀Cl₂N₂O₂₃
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetCa2+ indicators
      Purity≥95% by HPLC
      Physicochemical Information
      Cell permeableN
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      FLUO 3/AM - CAS 121714-22-5 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      FLUO 3/AM - CAS 121714-22-5 - Calbiochem Certificates of Analysis

      TitleLot Number
      343242

      References

      Reference overview
      Caputo, C., and Bolanos, P. 1994. J. Physiol. (London) 481, 119.
      Schumann, M.A., et al. 1993. J. Biol. Chem. 268, 2134.
      Eberhard, M., and Erne, P. 1989. Biochem. Biophys. Res. Commun. 163, 309.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Fluorescent Calcium Probes Technical Bulletin
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-December-2018 JSW
      Synonyms1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)]-2-(2ʹ-amino-5ʹ-methylphenoxy)ethane-N,N,Nʹ,Nʹ-tetraacetic Acid Pentaacetoxymethyl Ester
      DescriptionMembrane-permeable version of FLUO 3 (Cat. No. 343244). Cleavage of FLUO 3/AM by cytosolic esterases traps the indicator in the cell. FLUO 3 is almost nonfluorescent at resting calcium levels, but fluorescence levels increase 40-fold upon calcium binding. Unlike all other Ca2+ indicators, FLUO 3/AM is nonfluorescent until it is hydrolyzed by the cell. Since its excitation maximum is at 506 nm, this compound can be used in conjunction with caged compounds, which are released by UV light. Its relatively high Kd for Ca2+ facilitates the measurement of higher peaks of Ca2+ transients than is possible with FURA 2. FLUO 3 has an excitation maximum of 506 nm and an emission maximum of 526 nm in the presence of high and low calcium concentrations.
      FormReddish orange solid
      Recommended reaction conditionsThe FLUO 3/AM DMSO stock solution should be diluted to 1-5 µM in a physiological buffer. In some cases, use of the non-ionic detergent PLURIONIC® F-127, PROTEIN GRADE® (Cat. No. 540025) at 0.025-0.125% will assist in the dissolution of the dye in aqueous buffers. Cells are normally incubated with the dye for 20-60 min at 20-37°C.
      CAS number121714-22-5
      Chemical formulaC₅₁H₅₀Cl₂N₂O₂₃
      Structure formulaStructure formula
      Purity≥95% by HPLC
      SolubilityDMF and DMSO (5 mM)
      Storage -20°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesCaputo, C., and Bolanos, P. 1994. J. Physiol. (London) 481, 119.
      Schumann, M.A., et al. 1993. J. Biol. Chem. 268, 2134.
      Eberhard, M., and Erne, P. 1989. Biochem. Biophys. Res. Commun. 163, 309.