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SCT154 Firefly Luciferase Assay

Flash-type luciferase assay designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity.

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SCT154
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCT154
      DescriptionFirefly Luciferase Assay
      OverviewFirefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues. Firefly luciferase is a 62,000 Dalton protein, which is active as a monomer and does not require subsequent processing for its activity. The enzyme catalyzes ATP-dependent D-luciferin oxidation to oxyluciferin, producing light emission centered at 560 nm. Light emitted from the reaction is directly proportional to the number of luciferase enzyme molecules.

      The Firefly Luciferase Assay is designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity. This is a flash-type luminescence assay that requires signal to be measured immediately after adding working solution to samples. The luminescence signal decays over the course of about 10 minutes of reaction time, although signal half-life may vary depending on luciferase expression levels.
      Alternate Names
      • Firefly Luciferase Assay
      • Bioluminescent Assay
      References
      Product Information
      Components
      • 1) 5X Firefly Luciferase Lysis Buffer (CS224525): 2 X 15 mL
      • 2) Firefly Luciferase Assay Buffer 2.0 (CS224585): 100 mL
      • 3) D-Luciferin (CS224519): 2 X 10 mg
      Detection methodChemiluminescence
      HS Code3822 19 90
      Presentationliquid
      Applications
      ApplicationFlash-type luciferase assay designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity.
      Key Applications
      • Cell Based Assays
      Application NotesFirefly Luciferase Assay
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore Firefly Luciferase Assay at -80°C. Firefly Luciferase Assay Buffer 2.0 is stable at -20°C for three months and at –80°C for at least six months from date of receipt. The other kit components are stable at -20°C for at least six months from date of receipt. Kit components and D-luciferin stock solutions in water are stable to at least 5 freeze-thaw cycles.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCT154 04054839566066