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361524 GSK-3β, His•Tag®, Human, Recombinant, E. coli

GSK-3β, His-Tag®, Human, Recombinant, E. coli, is a dual specificity kinase that plays important roles in insulin- and Wnt-mediated signaling. It is inactivated by phosphorylation at Ser9.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: GSK-3β, Tau Protein Kinase I, TPK I

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361524
  
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      Overview

      Replacement Information
      Description
      OverviewRecombinant, human GSK-3β fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. A dual specificity kinase that plays important roles in insulin- and Wnt-mediated cellular signalings. In AD (Alzheimer’s disease), GSK3β has a major role in microtubule stability by its ability to phosphorylate the microtubule associated protein tau. Its known substrates include glycogen synthase, p90rsk, Tau, c-Jun, and CREB. MW: 53,000-56,000.
      Catalogue Number361524
      Brand Family Calbiochem®
      SynonymsGSK-3β, Tau Protein Kinase I, TPK I
      References
      ReferencesHarwood, A., and Braga, V.M. 2003. Nat. Cell Biol. 5, 275.
      Bhat, R.V., and Budd, S.L. 2002. Neurosignals 11, 251.
      Product Information
      FormSolid
      FormulationLyophilized from 0.1 M NH₄CO₃.
      Quality LevelMQ100
      Applications
      ApplicationGSK-3β, His-Tag®, Human, Recombinant, E. coli, is a dual specificity kinase that plays important roles in insulin- and Wnt-mediated signaling. It is inactivated by phosphorylation at Ser9.
      Biological Information
      Biological activity1 µg GSK-3β maximally phosphorylates 100 ng Tau protein in 30 min in an in vitro kinase assay at 30°C, pH 7.5
      Purity≥90% by SDS-PAGE
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      361524 0

      Documentation

      GSK-3β, His•Tag®, Human, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      GSK-3β, His•Tag®, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      361524

      References

      Reference overview
      Harwood, A., and Braga, V.M. 2003. Nat. Cell Biol. 5, 275.
      Bhat, R.V., and Budd, S.L. 2002. Neurosignals 11, 251.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-January-2009 JSW
      SynonymsGSK-3β, Tau Protein Kinase I, TPK I
      DescriptionRecombinant, human GSK-3β fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. Glycogen synthase kinase-3 (GSK-3) is a serine/threonine kinase that was first characterized by its role in intermediary metabolism. GSK-3β is a dual-specificity kinase that is important role in insulin- and Wnt-mediated cellular signaling events. In Alzheimer's disease GSK-3β is reported to affect microtubule stability via the phosphorylation of microtubule associated protein, Tau. Other known substrates include glycogen synthase, p90rsk, c-Jun, and CREB. MW: 53,000-56,000
      FormSolid
      FormulationLyophilized from 0.1 M NH₄CO₃.
      Recommended reaction conditions25 mM Tris-HCl, pH 7.5, 5 mM β-glycerophosphate, 12 mM MgCl2, 2 mM DTT, 100 µM Na3VO4, 200 µM ATP, 30°C.
      Purity≥90% by SDS-PAGE
      Biological activity1 µg GSK-3β maximally phosphorylates 100 ng Tau protein in 30 min in an in vitro kinase assay at 30°C, pH 7.5
      SolubilityReconstitute in sterile PBS to a final concentration of 1 mg/ml.
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.
      Toxicity Standard Handling
      ReferencesHarwood, A., and Braga, V.M. 2003. Nat. Cell Biol. 5, 275.
      Bhat, R.V., and Budd, S.L. 2002. Neurosignals 11, 251.