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368044 Granzyme A, Human, Recombinant, E. coli

Granzyme A, Human, Recombinant, E. coli, is a trypsin-like serine protease secreted by cytotoxic T cells and natural killer cells. Induces cell death through caspase-independent mechanism.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: GzmA

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368044
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Overview

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368044-10UG
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      		 Plastic ampoule 10 μg
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      Description
      OverviewRecombinant, human granzyme A expressed in E. coli. Granzyme A is a trypsin-like serine protease secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. A proapoptotic enzyme that induces cell death through caspase-independent mechanism, causing single-stranded DNA nicking and other apoptotic features including nuclear fragmentation, chromatin condensation, loss of mitochondrial potential, and membrane blebbing. Granzyme A is also known to bind to and cleave nucleolin and activate the thrombin receptor on neuronal cells and astrocytes. It also cleaves and activates interleukin-1β. Note: The M.W. is that of a homodimer.
      Catalogue Number368044
      Brand Family Calbiochem®
      SynonymsGzmA
      References
      ReferencesFan, Z., et al. 2002. Mol. Cell. Biol. 22, 2810.
      Russell, J.H., and Ley, T. J. 2002. Annu. Rev. Immunol. 20, 323.
      Beresford, P.J., et al. 2001. J. Biol. Chem. 276, 43285.
      Simon, M.M., and Kramer, M.D. 1994. Methods Enzymol. 244, 68.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that hydrolyzes 1 nmol Z-Lys-SBzl per min 25°C, pH 8.0.
      EC number3.4.21.78
      FormLiquid
      FormulationIn 520 mM NaCl, 50 mM MES, 10% glycerol, pH 6.0.
      Quality LevelMQ100
      Applications
      ApplicationGranzyme A, Human, Recombinant, E. coli, is a trypsin-like serine protease secreted by cytotoxic T cells and natural killer cells. Induces cell death through caspase-independent mechanism.
      Biological Information
      Specific Activity≥100 units/µg protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      368044-10UG 04055977192117

      Documentation

      Granzyme A, Human, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      Granzyme A, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      368044

      References

      Reference overview
      Fan, Z., et al. 2002. Mol. Cell. Biol. 22, 2810.
      Russell, J.H., and Ley, T. J. 2002. Annu. Rev. Immunol. 20, 323.
      Beresford, P.J., et al. 2001. J. Biol. Chem. 276, 43285.
      Simon, M.M., and Kramer, M.D. 1994. Methods Enzymol. 244, 68.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-July-2008 RFH
      SynonymsGzmA
      DescriptionRecombinant, human granzyme A expressed in E. coli. Granzyme A is a trypsin-like serine protease found in the cytotoxic granules and secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A is a pro-apoptotic enzyme that induces cell death through a caspase-independent mechanism. Granzyme A-induced apoptosis results in single-stranded DNA nicking, nuclear fragmentation, chromatin condensation, loss of mitochondrial potential, and membrane blebbing. Granzyme A is also known to bind to and cleave nucleolin, activate the thrombin receptor on neuronal cells and astrocytes, cleave and activate interleukin-1β, and cleave putative HLA-associated protein II (PHAP II). Recombinant human Granzyme A expressed in E. coli.
      FormLiquid
      FormulationIn 520 mM NaCl, 50 mM MES, 10% glycerol, pH 6.0.
      EC number3.4.21.78
      Specific activity≥100 units/µg protein
      Unit definitionOne unit is defined as the amount of enzyme that hydrolyzes 1 nmol Z-Lys-SBzl per min 25°C, pH 8.0.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesFan, Z., et al. 2002. Mol. Cell. Biol. 22, 2810.
      Russell, J.H., and Ley, T. J. 2002. Annu. Rev. Immunol. 20, 323.
      Beresford, P.J., et al. 2001. J. Biol. Chem. 276, 43285.
      Simon, M.M., and Kramer, M.D. 1994. Methods Enzymol. 244, 68.