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SCT065 Green-AlDeSense™ ALDH1A1 Live Cell Dye

ALDH1A1 isoform specific live cell dye which labels cancer stem cells for fluorescent microscopy, flow cytometry and in vivo applications.

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SCT065
1/Kit  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCT065
      DescriptionGreen-AlDeSense™ ALDH1A1 Live Cell Dye
      OverviewCancer stem cells (CSCs) have an increased capacity to activate antiapoptotic/prosurvival pathways as well as overexpressing ATP-binding cassette transporters which act as potent efflux pumps for potential chemotherapeutics. Aldehyde dehydrogenases (ALDH), in particular the ALDH1A1 isoform, is believed to be a reliable marker of CSCs across many cancer types, including prostate, lung, breast, esophageal, and ovarian cancers. ALDH1A1 is associated with increased chemotherapy resistance and poor clinical outcome and is an established stem cell marker.

      Current methods to identify ALDH in living cells are limited by cross-reactivity with several ALDH isoforms producing difficulties in data analysis and prognosis. Chan, et al. have created a ALDH1A1 isoform specific live cell dye termed Green-AlDeSense™ which labels cancer stem cells and exhibits a 20-fold fluorescent enhancement when bound to ALDH1A1. The dye identifies ALDH1A1 positive CSCs using live cell imaging, flow cytometry and in vivo studies.

      Spectral Properties: absmax: 496 nm, emmax: 516 nm.

      Reference

      1) Chan, et al. Surveillance of Cancer Stem Cell Plasticity Using an Isoform-Selective Fluorescent Probe for Aldehyde Dehydrogenase 1A1. ACS Cent Sci. 2018 Aug 22;4(8):1045-1055.
      References
      Product Information
      Components
      • The Green-AlDeSense™ ALDH1A1 Live Cell Dye Kit (SCT065) contains:
      • 1) Ctrl-Green-AlDeSense™ Dye (CS226256), 100 µg. Store at -20°C. Protect from light. MW: 380.3 g/mol.
      • 2) Green-AlDeSense™ Dye (CS226257), 100 µg. Store at -20°C. Protect from light. MW: 438.4 g/mol.
      Detection methodFluorescence
      Applications
      ApplicationALDH1A1 isoform specific live cell dye which labels cancer stem cells for fluorescent microscopy, flow cytometry and in vivo applications.
      Key Applications
      • Flow Cytometry
      • Fluorescence
      • Cell Based Assays
      Biological Information
      Gene Symbol
      • ALDH1A1
      Molecular WeightCtrl-Green-AlDeSense™ Dye: 380.3 g/mol.
      Green-AlDeSense™ Dye: 438.4 g/mol.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceColor: Orange
      Form: Solid
      Solubility: DMSO: >10 mg/mL
      Purity: ≥ 80.0%
      Elemental Analysis/Identification: Conforms
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20ﾟC. Protect from light.
      Packaging Information
      Material Size1/Kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCT065 04061841849773