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375230 HGF, Mouse, Recombinant

HGF, Mouse, Recombinant Recombinant, consists of disulfide-linked heterodimeric α (Gln33 to Arg495) and β (Val496 to Leu728) chains expressed in murine myeloma NS0 cell line.

HGF, Mouse, Recombinant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Hepatopoietin A

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375230
  
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      Overview

      Replacement Information

      Key Spec Table

      Purity
      ≥95% by SDS-PAGE as seen by a 60 kDa (α chain) and a 33 kDa (β chain) bands under reducing conditions.
      Description
      Overview

      This product has been discontinued.



      Recombinant, mouse hepatocyte growth factor (HGF) consisting of disulfide-linked heterodimeric α (Gln33 to Arg495) and β (Val496 to Leu728) chains expressed in murine myeloma NS0 cell line.
      Catalogue Number375230
      Brand Family Calbiochem®
      SynonymsHepatopoietin A
      References
      ReferencesCorso, S., et al. 2005. Trends Mol. Med. 11, 284.
      Karihaloo, A., et al. 2005. Nephron Exp. Nephrol. 100, e40.
      Urbanek, K., et al. 2005. Circ. Res. 97, 663.
      Hammond, D.E., et al. 2004. Curr. Top. Microbiol. Immunol. 286, 21.
      Rosario, M. and W. Birchmeier. 2004. Dev. Cell 7, 3.
      Gheradi, E., et al. 2003. Proc. Natl. Acad. Sci. 100, 12039.
      Chiu, S.J., et al. 2002. J. Biomed. Sci. 9, 261.
      Tanaka, Y., et al. 2002. Biochem. Biophys. Res. Commun. 299, 472.
      Maeshima, A., et al. 2000. Kid. Int. 58, 1511.
      Lesk, A.M. and W.D. Fordham. 1996. J. Mol. Biol. 258, 501.
      Saelman, E.U.M., et al. 1995. J. Cell Sci. 108, 3531.
      Mineo, R., et al. 1994. Endocrinology 145, 4355.
      Mizuno, K., et al. 1994. J. Biol. Chem. 269, 1131.
      Sasaki, M., et al. 1994. Biochem. Biophys. Res. Commun. 199, 772.
      Montesano, R., et al. 1991. Cell 67, 901.
      Product Information
      FormSolid
      FormulationLyophilized from 0.2 µm sterile-filtered PBS, BSA.
      Quality LevelMQ100
      Applications
      ApplicationHGF, Mouse, Recombinant Recombinant, consists of disulfide-linked heterodimeric α (Gln33 to Arg495) and β (Val496 to Leu728) chains expressed in murine myeloma NS0 cell line.
      Biological Information
      Biological activityCell Proliferation:
      ED₅₀ = 10-30 ng/ml as measured in a cell proliferation assay using IMCD-3 mouse epithelial cells.

      Functional ELISA
      KD <0.2 nM as measured by binding to C-Met (HGF Receptor).
      Purity≥95% by SDS-PAGE as seen by a 60 kDa (α chain) and a 33 kDa (β chain) bands under reducing conditions.
      Physicochemical Information
      ContaminantsEndotoxin: <1.0 EU/µg protein
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      375230 0

      Documentation

      HGF, Mouse, Recombinant SDS

      Title

      Safety Data Sheet (SDS) 

      HGF, Mouse, Recombinant Certificates of Analysis

      TitleLot Number
      375230

      References

      Reference overview
      Corso, S., et al. 2005. Trends Mol. Med. 11, 284.
      Karihaloo, A., et al. 2005. Nephron Exp. Nephrol. 100, e40.
      Urbanek, K., et al. 2005. Circ. Res. 97, 663.
      Hammond, D.E., et al. 2004. Curr. Top. Microbiol. Immunol. 286, 21.
      Rosario, M. and W. Birchmeier. 2004. Dev. Cell 7, 3.
      Gheradi, E., et al. 2003. Proc. Natl. Acad. Sci. 100, 12039.
      Chiu, S.J., et al. 2002. J. Biomed. Sci. 9, 261.
      Tanaka, Y., et al. 2002. Biochem. Biophys. Res. Commun. 299, 472.
      Maeshima, A., et al. 2000. Kid. Int. 58, 1511.
      Lesk, A.M. and W.D. Fordham. 1996. J. Mol. Biol. 258, 501.
      Saelman, E.U.M., et al. 1995. J. Cell Sci. 108, 3531.
      Mineo, R., et al. 1994. Endocrinology 145, 4355.
      Mizuno, K., et al. 1994. J. Biol. Chem. 269, 1131.
      Sasaki, M., et al. 1994. Biochem. Biophys. Res. Commun. 199, 772.
      Montesano, R., et al. 1991. Cell 67, 901.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision21-June-2013 JSW
      SynonymsHepatopoietin A
      DescriptionRecombinant, mouse hepatocyte growth factor (HGF) consisting of disulfide-linked heterodimeric α (Gln33 to Arg495) and β (Val496 to Leu728) chains expressed in murine myeloma NS0 cell line.
      FormSolid
      FormulationLyophilized from 0.2 µm sterile-filtered PBS, BSA.
      Purity≥95% by SDS-PAGE as seen by a 60 kDa (α chain) and a 33 kDa (β chain) bands under reducing conditions.
      ContaminantsEndotoxin: <1.0 EU/µg protein
      Biological activityCell Proliferation:
      ED₅₀ = 10-30 ng/ml as measured in a cell proliferation assay using IMCD-3 mouse epithelial cells.

      Functional ELISA
      KD <0.2 nM as measured by binding to C-Met (HGF Receptor).
      SolubilityReconstitute to 10 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesCorso, S., et al. 2005. Trends Mol. Med. 11, 284.
      Karihaloo, A., et al. 2005. Nephron Exp. Nephrol. 100, e40.
      Urbanek, K., et al. 2005. Circ. Res. 97, 663.
      Hammond, D.E., et al. 2004. Curr. Top. Microbiol. Immunol. 286, 21.
      Rosario, M. and W. Birchmeier. 2004. Dev. Cell 7, 3.
      Gheradi, E., et al. 2003. Proc. Natl. Acad. Sci. 100, 12039.
      Chiu, S.J., et al. 2002. J. Biomed. Sci. 9, 261.
      Tanaka, Y., et al. 2002. Biochem. Biophys. Res. Commun. 299, 472.
      Maeshima, A., et al. 2000. Kid. Int. 58, 1511.
      Lesk, A.M. and W.D. Fordham. 1996. J. Mol. Biol. 258, 501.
      Saelman, E.U.M., et al. 1995. J. Cell Sci. 108, 3531.
      Mineo, R., et al. 1994. Endocrinology 145, 4355.
      Mizuno, K., et al. 1994. J. Biol. Chem. 269, 1131.
      Sasaki, M., et al. 1994. Biochem. Biophys. Res. Commun. 199, 772.
      Montesano, R., et al. 1991. Cell 67, 901.