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SCC129 HT-22 Mouse Hippocampal Neuronal Cell Line

≥1X10⁶ cells/vial   
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      Key Spec Table

      Key Applications
      Neurotoxicity, Cell Based Assays
      Catalogue NumberSCC129
      DescriptionHT-22 Mouse Hippocampal Neuronal Cell Line
      OverviewHT-22 is an immortalized mouse hippocampal cell line subcloned from the HT-4 cell line (1). The parental HT-4 cell line was derived from the immortalization of mouse neuronal tissues with a temperature sensitive SV40 T-antigen (2). HT-22 is highly sensitive to glutamate and is thus frequently used as a model system to study glutamate-induced toxicity in neuronal cells.

      1. Davis JB and Maher P (1994) Protein kinase C activation inhibits glutamate-induced cytotoxicity in a neuronal cell line. Brain Res 652(1): 169-173.

      2. Morimoto BH and Koshland DE Jr. (1990) Induction and expression of long- and short-term neurosecretory potentiation in a neural cell line. Neuron 5(6): 875-880.
      Alternate Names
      • HT22 Cell Line
      Background InformationGlutamate is a major excitatory neurotransmitter used as a signaling molecule between nerve cells and is involved in most aspects of normal brain function including cognition, learning and memory. Various glutamate receptors are found throughout the brain and include NMDA, AMPA/kainate and metabotropic receptors (mGluR). Normal glutamate levels are essential for normal brain function, however high levels may result in over-excitation of nerve cells, leading to eventual cell damage and/or cell death. Glutamate-induced cytotoxicity has been implicated in neurodegenerative disorders such as Alzheimer’s disease, Huntington’s disease and Parkinson’s disease along with other conditions including spinal cord injury and multiple sclerosis. Therapies that may inhibit or reduce the effects of glutamate activity are thus of great therapeutic interest.
      Product Information
      Quality LevelMQ100
      ApplicationHT-22 mouse neuronal cell line is a valuable cell model for studies of glutamate-induced toxicity in neuronal cells.
      Key Applications
      • Neurotoxicity
      • Cell Based Assays
      Application NotesEMD Millipore has a limited license to sell this product for research use only, and not for any commercial uses. Excluded commercial uses include without limitation manufacturing, providing a service, therapeutic, diagnostic and prophylactic uses, and any other commercial uses. Use of this product by a purchaser for any purpose other than for research is unauthorized and prohibited.

      Inquiries about licensing for commercial or other uses should be directed to:

      Office of Technology Development
      The Salk Institute for Biological Studies
      10010 North Torrey Pines Road
      La Jolla, CA 92037
      Phone: (858) 453-4100 extension 1278
      Biological Information
      Cell Line Type
      • Neural Lineage Cells
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X106 viable cells.
      • Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services.
      • Cells are negative for mycoplasma contamination.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information