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SCT212-30nmol HaloTag® AcidiFluor™ Orange Ligand

Live Cell imaging probe for acidic pH. It shows weak fluorescence at neutral pH, but shows strong fluorescence at acidic pH.

SCT212-30nmol
30 nmol  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCT212-30nmol
DescriptionHaloTag® AcidiFluor™ Orange Ligand
OverviewHaloTag® AcidiFluorTM ORANGE is an acidic pH indicator. It consists of AcidiFluor ORANGE dye fused with HaloTag® ligand, which forms a covalent bond with Haotag. It shows quite weak fluorescence at neutral pH, but shows strong fluorescence at acidic pH. Since it is cell impermeable reagent, it can be used to label membrane-associated HaloTag fused proteins just by mixing and then monitoring pH changes. Relatively photostable fluorescence of AcidiFluor™ ORANGE enables time lapse
imaging of pH change and tracking of endocytic/exocytic vesicles for long-time period.

Spectral Properties
Absorbance (max): 520nm
Emission (max): 565nm

References
Isa M et al. High-Throughput Screening System To Identify Small Molecules That Induce Internalization and Degradation of HER2. ACS Chem. Biol. 2014, 9, 10, 2237-2241.

HaloTag® is a registered tradeMark of Promega Corporation.
AcidiFluor™ is a trademark of Goryo Chemical Inc.
Alternate Names
  • HaloTag® AcidiFluor™ORANGE Ligand, 30 nmol
References
Product Information
Detection methodFluorescence
PresentationLyophilized
Applications
ApplicationLive Cell imaging probe for acidic pH. It shows weak fluorescence at neutral pH, but shows strong fluorescence at acidic pH.
Key Applications
  • Cell Based Assays
  • Cell Surface Receptor Assays
Application NotesLive cell fluorescent imaging. Orange fluorescence.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssurancePurity: ≥ 90% (LC)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore HaloTag® AcidiFluor™ Orange Ligand at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Packaging Information
Material Size30 nmol
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCT212-30nmol 0