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PF078 Heparin Binding Epidermal Growth Factor, Human, Recombinant, S. frugiperda

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: HB-EGF

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PF078
  
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      Overview

      Replacement Information

      Key Spec Table

      Purity
      ≥97% by SDS-PAGE
      Description
      OverviewRecombinant, human heparin binding epidermal growth factor expressed in S. frugiperda insect cells. The mature HB-EGF is generated by the removal of the 62 amino acid (~9.5 kDa) signal. Protein is heterogeneously O-glycosylated and migrates as an ~12 kDa protein in SDS-PAGE. Titrate the protein for optimal results in individual systems.
      Catalogue NumberPF078
      Brand Family Calbiochem®
      SynonymsHB-EGF
      References
      ReferencesDavis-Fleischer, K.M. and Besner, G.E. 1998. Front. Biosci. 3, D288.
      Ouchi, N., et al. 1997. Biochem. J. 328, 923.
      Raab, G. and Klagsbrun, M. 1997. Biochim. Biophys. Acta 1333, F179.
      Suzuki, M., et al. 1997. J. Biol. Chem. 272, 31730.
      Nakata, A., et al. 1996. Circulation 94, 2778.
      Miyagawa, J., et al. 1995. J. Clin. Invest. 95, 404.
      Higashiyama, S., et al. 1991. Science 251, 936.
      Product Information
      FormSolid
      FormulationLyophilized from a sterile-filtered PBS solution, 50 µg of BSA/µg of cytokine, pH 7.4.
      Quality LevelMQ100
      Applications
      Data setup Error. Pagelet <collection_feature_application_id-PROLIF> not found.
      Application NotesProliferation studies
      Biological Information
      Biological activityEC₅₀ of 2-5 ng/ml as measured by its ability to stimulate ³H-thymidine incorporation in the EGF-responsive fibroblast cell line, Balb/3T3
      Purity≥97% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxins: ≤1 EU per µg cytokine
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PF078 0

      Documentation

      Heparin Binding Epidermal Growth Factor, Human, Recombinant, S. frugiperda SDS

      Title

      Safety Data Sheet (SDS) 

      Heparin Binding Epidermal Growth Factor, Human, Recombinant, S. frugiperda Certificates of Analysis

      TitleLot Number
      PF078

      References

      Reference overview
      Davis-Fleischer, K.M. and Besner, G.E. 1998. Front. Biosci. 3, D288.
      Ouchi, N., et al. 1997. Biochem. J. 328, 923.
      Raab, G. and Klagsbrun, M. 1997. Biochim. Biophys. Acta 1333, F179.
      Suzuki, M., et al. 1997. J. Biol. Chem. 272, 31730.
      Nakata, A., et al. 1996. Circulation 94, 2778.
      Miyagawa, J., et al. 1995. J. Clin. Invest. 95, 404.
      Higashiyama, S., et al. 1991. Science 251, 936.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-September-2008 RFH
      SynonymsHB-EGF
      ApplicationProliferation studies
      DescriptionRecombinant, human heparin binding epidermal growth factor expressed in S. frugiperda insect cells. The mature HB-EGF is a human recombinant protein whose DNA sequence encoding the N-terminal 148 amino acid residues of human HB-EGF precursor was expressed in Sf 21 insect cells using a baculovirus expression system. The mature recombinant protein (86 amino acids) generated by the removal of the 62 amino acid residue signal and propeptide has a predicted molecular mass of ~9.5 kDa. The recombinant protein is heterogeneously O-glycosylated and migrates as an ~12 kDa protein in SDS-PAGE. Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a ~22 kDa O-glycosylated protein that is a potent mitogen and chemoattractant for vascular smooth muscle cells, fibroblasts, and epithelial cells but not endothelial cells. The natural protein has an apparent molecular mass of ~19-23 kDa and exists in multiple forms as a result of heterogeneous O-glycosylation and/or N-terminal truncation. HB-EGF is synthesized as a membrane-anchored precursor (proHB-EGF) that is proteolytically cleaved to release the soluble mature growth factor. The two forms are active as juxtacrine and paracrine/autocrine growth factors respectively. Recent studies show MMP-3 as one of the enzymes that cleaves HB-EGF implicating a role for MMP-3 in the regulation of HB-EGF from being a juxtacrine to a paracrine/autocrine factor. HB-EGF activates two EGF receptor subtypes, HER1/ErbB1, and HER4 and binds to heparan sulfate proteoglycan. The proHB-EGF is postulated as the high affinity receptor for diphtheria toxin (DT). Recent evidence suggests that the coexpression of proHB-EGF and CD9 on macrophages may strongly promote the development of atherosclerosis by a juxtacrine mechanism. Transcription of HB-EGF can be induced in vascular endothelial cells as well as aortic smooth muscle cells which may also play an important role in the pathogenesis of atherosclerosis. Useful for proliferation studies.
      FormSolid
      FormulationLyophilized from a sterile-filtered PBS solution, 50 µg of BSA/µg of cytokine, pH 7.4.
      Purity≥97% by SDS-PAGE
      ContaminantsEndotoxins: ≤1 EU per µg cytokine
      Biological activityEC₅₀ of 2-5 ng/ml as measured by its ability to stimulate ³H-thymidine incorporation in the EGF-responsive fibroblast cell line, Balb/3T3
      SolubilityReconstitute in PBS to ≥1 µg/ml containing ≥0.1%HSA or BSA.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesDavis-Fleischer, K.M. and Besner, G.E. 1998. Front. Biosci. 3, D288.
      Ouchi, N., et al. 1997. Biochem. J. 328, 923.
      Raab, G. and Klagsbrun, M. 1997. Biochim. Biophys. Acta 1333, F179.
      Suzuki, M., et al. 1997. J. Biol. Chem. 272, 31730.
      Nakata, A., et al. 1996. Circulation 94, 2778.
      Miyagawa, J., et al. 1995. J. Clin. Invest. 95, 404.
      Higashiyama, S., et al. 1991. Science 251, 936.