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377853 Hirudin, Hirudo medicinalis, Recombinant, Saccharomyces cerevisiae - CAS 8001-27-2 - Calbiochem

Hirudin, Hirudo medicinalis, Recombinant, Saccharomyces cerevisiae, CAS 8001-27-2, is an anticoagulant that inactivates thrombin by blocking substrate binding groups.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Primary Target: Has highly specific antithrombin properties
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      Overview

      Replacement Information

      Key Spec Table

      CAS #
      8001-27-2
      Description
      OverviewHV1 form devoid of sulfate residue at Tyr63. Has highly specific antithrombin properties. Inactivates thrombin by blocking substrate binding groups.
      Catalogue Number377853
      Brand Family Calbiochem®
      References
      Product Information
      CAS number8001-27-2
      Unit of DefinitionOne unit is defined as the amount of hirudin that will neutralize 1 NIH unit of thrombin (fibrinogen assay) at 37°C, pH 7.5.
      ATP CompetitiveN
      FormLyophilized
      ReversibleN
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetHas highly specific antithrombin properties
      Specific Activity≥10,000 antithrombin units (ATU)/mg protein
      Physicochemical Information
      Cell permeableN
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSMR8440000
      Safety Information
      R PhraseR: 20/21/22

      Harmful by inhalation, in contact with skin and if swallowed.
      S PhraseS: 36

      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      377853 0

      Documentation

      Hirudin, Hirudo medicinalis, Recombinant, Saccharomyces cerevisiae - CAS 8001-27-2 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Hirudin, Hirudo medicinalis, Recombinant, Saccharomyces cerevisiae - CAS 8001-27-2 - Calbiochem Certificates of Analysis

      TitleLot Number
      377853
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision12-May-2008 RFH
      DescriptionHV1 form devoid of the sulfate residue at Tyr63. Exhibits highly specific antithrombin properties. Inactivates thrombin by blocking substrate binding groups.
      FormLyophilized
      Recommended reaction conditions
      Protocol for the Assay of Hirudin Reagents: 1. 100 NIH units/ml thrombin 2. 9 mg/ml isotonic saline (0.9%) 3. 0.5% fibrinogen in isotonic saline 4. hirudin reconstituted with 1.0 ml of distilled H2O, isotonic saline, or other suitable buffer Assay: 1. Add 10-100 µl of the sample to be assayed to 200 µl of 0.5% fibrinogen solution and mix well. 2. Add 5 µl aliquots of a standardized thrombin solution (100 NIH units/ml) at one min intervals with mixing at 20°C. 3. The end-point is reached when a fibrin clot appears within one minute. Calculate the number of antithrombin units (ATU) of hirudin present in the solution. Note: This protocol is meant to serve only as a guideline. It may need to be modified for specific applications.
      CAS number8001-27-2
      RTECSMR8440000
      Specific activity≥10,000 antithrombin units (ATU)/mg protein
      Unit definitionOne unit is defined as the amount of hirudin that will neutralize 1 NIH unit of thrombin (fibrinogen assay) at 37°C, pH 7.5.
      SolubilityH₂O. Reconstitute with 1 ml H₂O.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Harmful
      Merck USA index14, 4718