Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Description
Overview
A modified biotin conjugate in which biotin is linked to a bis-aryl hydrazone chromophore and aromatic succinimidyl ester via a long-chain PEG4 linker. The bis-aryl hydrazone chromophore allows for direct spectrophotometric quantitation of total incorporated biotin. The long-chain PEG4 linker preserves biotin/avidin affinity as well as increases solubility. The aromatic succinimidyl ester allows for higher efficiency modification of amines in aqueous buffers.
Catalogue Number
203119
Brand Family
Calbiochem®
Synonyms
NHS-Chromogenic Biotin
References
References
Steinberg-Tatman, G., et al. 2006. Bioconjug. Chem.17, 841. Kline, M.C., et al. 2005. J. Forensic Sci.50, 1. Kozlov, I.A., et al. 2004. Biopolymers73, 621. Chen, Y., et al. 2004. Chem. Commun.24, 2804. Kozlov, I.A., et al. 2004. Biopolymers73, 621. Zhong, X.B., et al. 2003. Proc. Natl. Acad. Sci. USA100, 11559. Hartmann, D.M., et al. 2002. Journal of Materials Research17, 473.
Steinberg-Tatman, G., et al. 2006. Bioconjug. Chem.17, 841. Kline, M.C., et al. 2005. J. Forensic Sci.50, 1. Kozlov, I.A., et al. 2004. Biopolymers73, 621. Chen, Y., et al. 2004. Chem. Commun.24, 2804. Kozlov, I.A., et al. 2004. Biopolymers73, 621. Zhong, X.B., et al. 2003. Proc. Natl. Acad. Sci. USA100, 11559. Hartmann, D.M., et al. 2002. Journal of Materials Research17, 473.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
07-April-2016 JSW
Synonyms
NHS-Chromogenic Biotin
Description
A modified biotin conjugate in which biotin is linked to a bis-aryl hydrazone chromophore and aromatic succinimidyl ester via a long-chain PEG4 linker. The bis-aryl hydrazone chromophore allows for direct spectrophotometric quantitation of total incorporated biotin. The long-chain PEG4 linker preserves biotin/avidin affinity as well as increases solubility. The aromatic succinimidyl ester allows for higher efficiency modification of amines in aqueous buffers.
Form
Solid
Chemical formula
C₃₈H₅₀N₈O₁₀S
Structure formula
Purity
≥90% by RP-HPLC
Solubility
DMF (50 mg/ml)
Storage
Protect from moisture +2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, purge with inert gas, aliquot, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity
Standard Handling
References
Steinberg-Tatman, G., et al. 2006. Bioconjug. Chem.17, 841. Kline, M.C., et al. 2005. J. Forensic Sci.50, 1. Kozlov, I.A., et al. 2004. Biopolymers73, 621. Chen, Y., et al. 2004. Chem. Commun.24, 2804. Kozlov, I.A., et al. 2004. Biopolymers73, 621. Zhong, X.B., et al. 2003. Proc. Natl. Acad. Sci. USA100, 11559. Hartmann, D.M., et al. 2002. Journal of Materials Research17, 473.
