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422425 Keratinocyte Growth Factor, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: rhKGF, rhFGF-7

422425
  
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      Overview

      Replacement Information

      Key Spec Table

      Purity
      ≥97% by SDS-PAGE
      Description
      Overview

      This product has been discontinued.





      Recombinant, human Keratinocyte growth factor expressed in E. coli. An epithelial cell-specific mitogen. KGF binding to its receptor is blocked by Suramin (Cat. No. 574625). Thought to mediate the hypothesized mesenchyme-driven proliferation of normal epithelial cells.
      Catalogue Number422425
      Brand Family Calbiochem®
      SynonymsrhKGF, rhFGF-7
      References
      ReferencesFinch, P.W., et al. 1996. Gastroenterology 110, 441.
      Marchese, C., et al. 1995. J. Exp. Med. 182, 1369.
      Siddiqi, I., et al. 1995. Biochem. Biophys. Res. Commun. 215, 309.
      Ron, D., et al. 1993. J. Biol. Chem. 268, 2984.
      Werner, S., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 6896.
      Finch, P.W., et al. 1989. Science 245, 752.
      Product Information
      CAS number148348-15-6
      FormLyophilized
      FormulationLyophilized from sterile-filtered PBS, 50 µg BSA/µg KGF.
      Applications
      Biological Information
      Biological activityED₅₀ = 15-25 ng/ml as measured by its ability to stimulate the proliferation of a monkey epithelial cell line, 4MBr-5
      Purity≥97% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: ≤100 pg/µg KGF
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/37/38

      Irritating to eyes, respiratory system and skin.
      S PhraseS: 26-36

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Irritant
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      422425 0

      Documentation

      Keratinocyte Growth Factor, Human, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Finch, P.W., et al. 1996. Gastroenterology 110, 441.
      Marchese, C., et al. 1995. J. Exp. Med. 182, 1369.
      Siddiqi, I., et al. 1995. Biochem. Biophys. Res. Commun. 215, 309.
      Ron, D., et al. 1993. J. Biol. Chem. 268, 2984.
      Werner, S., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 6896.
      Finch, P.W., et al. 1989. Science 245, 752.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-August-2008 RFH
      SynonymsrhKGF, rhFGF-7
      DescriptionRecombinant, human Keratinocyte growth factor expressed in E. coli. An epithelial cell-specific mitogen. KGF binding to its receptor is blocked by Suramin (Cat. No. 574625). Thought to mediate the hypothesized mesenchyme-driven proliferation of normal epithelial cells.
      FormLyophilized
      FormulationLyophilized from sterile-filtered PBS, 50 µg BSA/µg KGF.
      CAS number148348-15-6
      Purity≥97% by SDS-PAGE
      ContaminantsEndotoxin: ≤100 pg/µg KGF
      Biological activityED₅₀ = 15-25 ng/ml as measured by its ability to stimulate the proliferation of a monkey epithelial cell line, 4MBr-5
      SolubilityReconstitute to a concentration ≥10 µg/ml with sterile PBS containing at least 0.1% HSA or BSA.
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
      Toxicity Irritant
      ReferencesFinch, P.W., et al. 1996. Gastroenterology 110, 441.
      Marchese, C., et al. 1995. J. Exp. Med. 182, 1369.
      Siddiqi, I., et al. 1995. Biochem. Biophys. Res. Commun. 215, 309.
      Ron, D., et al. 1993. J. Biol. Chem. 268, 2984.
      Werner, S., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 6896.
      Finch, P.W., et al. 1989. Science 245, 752.