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SCM064SFM LX-2 Human Hepatic Stellate Serum-Free Media

Serum-Free media optimized to expand and culture LX-2 human hepatic stellate cells.

SCM064SFM
250 mL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCM064SFM
      DescriptionLX-2 Human Hepatic Stellate Serum-Free Media
      Alternate Names
      • HSC
      • Perisinusoidal cells
      • Ito cells
      • Hepatic lipocytes
      • Hepatic pericytes
      Background InformationLX-2 Human Hepatic Stellate Serum-Free Media (SCM064SFM) is a ready to use, serum-free culture medium specifically designed for the growth and proliferation of LX-2 human hepatic stellate cells (SCC064, SCC064SF).

      Hepatic stellate cells are a major cell type responsible for liver fibrosis following their activation into fibrogenic myofibroblast-like cells in diseases such as chronic alcoholism, hepatitis B and C, fatty liver disease, obesity, and diabetes. LX-2 cells, an immortalized human hepatic stellate cells, retain key features of primary hepatic stellate cells (HSCs) and have been extensively utilized in the study of liver fibrosis.

      SCM064SFM eliminates the reliance on FBS, providing a consistent and controlled environment for cell culture. This ensures reproducibility in experimental results and reduces variability often associated with serum-based media. The media is optimized to support the growth and proliferation of LX-2 cells, enabling researchers to conduct experiments with similar cell yields and viability compared with cells grown on FBS supplemented media. By eliminating the need for FBS, SCM064SFM significantly reduces the demand for animal-derived products, contributing to ethical research practices and animal welfare.
      References
      Product Information
      Components
      • 1) 250 mL LX-2 Human Hepatic Stellate Serum-Free Media: (Catalog No. SCC064SFM). Store in liquid nitrogen.
      Quality LevelMQ100
      Applications
      ApplicationSerum-Free media optimized to expand and culture LX-2 human hepatic stellate cells.
      Key Applications
      • Stem Cell Culture
      • Cell Culture
      Biological Information
      HostHuman
      Cell Line Type
      • Hepatocyte Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceAppearance (Color): Clear, Red Liquid
      pH: 7.2 – 7.8
      Osmolality: 289-310 mOsm
      Sterility Tested: No Growth/Pass
      Endotoxin: <2 EU/mL
      Mycoplasma: Negative
      Functional Assay: Thaw and culture of LX-2 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsUpon receipt store at -20˚C. When ready to use, thaw overnight at 2-8˚C. Once thawed, mix thoroughly. Use immediately and store at 2-8˚C for up to 3-4 weeks. Multiple freeze/thaw cycles are not recommended.
      Packaging Information
      Material Size250 mL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCM064SFM 04065272306232