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HCYTA-60K MILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panel A - Immunology Multiplex Assay

Simultaneously analyze up to 48 multiple cytokine, chemokine, and growth factor biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.

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Research Category: Inflammation & Immunology Detection Methods: Luminex xMAP Research Sub-Category: Inflammation & Autoimmune Mechanisms, Inflammation, Infectious Diseases, Cytokines & Cytokine Receptors, Growth Factors & Receptors, Oncology, Obesity, Metabolic Disorders Key Applications: Multiplexing
HCYTA-60K

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Overview

Replacement Information

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This panel is available for custom premix. Talk to your Protein Specialist today!

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsMultiplexing Ordering RequirementsDetection Methods
sCD40L, EGF, Eotaxin, FGF-2, Flt-3 ligand, Fractalkine, G-CSF, GM-CSF, GROα, IFNα2, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-17E/IL-25, IL-17F, IL-18, IL-22, IL-27, IP-10, MCP-1, MCP-3, M-CSF, MDC (CCL22), MIG, MIP-1α, MIP-1β, PDGF-AA, PDGF-AB/BB, RANTES, TGFα, TNFα, TNFβ, VEGF-AHMplexNote: Sample type must be selected when configuring this kit. RANTES cannot be plexed with other analytes for serum/plasma and is not intended to be plexed into the premix kits when measuring serum/plasma samples. For questions, contact Technical Service.Luminex xMAP
Description
Catalogue NumberHCYTA-60K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panel A - Immunology Multiplex Assay
OverviewMILLIPLEX® offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the verification process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability).

Each MILLIPLEX® panel and kit includes:
• Quality controls (QCs) to qualify assay performance
• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
• Optimized serum matrix to mimic native analyte environment in serum and plasma samples
• Detection antibody cocktails designed to yield consistent analyte profiles within panel

In addition each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility. The MILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panel A thus enables you to focus on the therapeutic potential of cytokines and the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.
Background InformationCytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses.“Cytokine” is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. Cytokines differ from hormones in that they act on a wider spectrum of target cells. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Growth factors are involved in the stimulation of target cell survival, proliferation, differentiation with effects on angiogenesis, vasculogenesis, cell migration, apoptosis, wound healing and embryogenesis.

Cytokine, chemokine and growth factor research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process, and disease states such as infectious disease, osteoarthritis, respiratory disease, IBD, sepsis, allergic reactions as well as neurologic, metabolic and cardiovascular disease and even cancer.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra-assay: <15%
  • Inter-assay: <20%
Multiplex Ordering RequirementsNote: Sample type must be selected when configuring this kit. RANTES cannot be plexed with other analytes for serum/plasma and is not intended to be plexed into the premix kits when measuring serum/plasma samples. For questions, contact Technical Service.
Panel TypeMAGNETIC Cytokines/Chemokines
Applications
ApplicationSimultaneously analyze up to 48 multiple cytokine, chemokine, and growth factor biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight or two-hour incubation assay.
• This assay requires 25 µL of neat plasma or serum (1:100 dilution for RANTES) or 25 μL cell culture supernatant per well.
• This kit may be used for the analysis of any or all of the Human Cytokine/Chemokine/Growth Factor Panel A analytes in human tissue/cell lysate and culture supernatant samples and serum or plasma samples.
• RANTES cannot be plexed with other cytokines in this panel due to a required 1:100 dilution of plasma/serum samples.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • sCD40L
  • EGF
  • Eotaxin
  • FGF-2
  • Flt-3 ligand
  • Fractalkine
  • G-CSF
  • GM-CSF
  • GROα
  • IFNα2
  • IFNγ
  • IL-1α
  • IL-1β
  • IL-1ra
  • IL-2
  • IL-3
  • IL-4
  • IL-5
  • IL-6
  • IL-7
  • IL-8
  • IL-9
  • IL-10
  • IL-12 (p40)
  • IL-12 (p70)
  • IL-13
  • IL-15
  • IL-17A
  • IL-17E/IL-25
  • IL-17F
  • IL-18
  • IL-22
  • IL-27
  • IP-10
  • MCP-1
  • MCP-3
  • M-CSF
  • MDC (CCL22)
  • MIG
  • MIP-1α
  • MIP-1β
  • PDGF-AA
  • PDGF-AB/BB
  • RANTES
  • TGFα
  • TNFα
  • TNFβ
  • VEGF-A
Cross ReactivityThere was no or negligible cross-reactivity observed between the antibodies for any single analyte and any of the other analytes in this panel, except IL-12 (p40). IL-12 (p40) exhibited a cross-reactivity of approximately 8% with IL-12 (p70). This is not unexpected since the p40 subunit is a constituent of IL-12 (p70).
Accuracy
  • 92% - 106%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities for individual cytokines, chemokines and growth factors.
Standard Curve Range
  • MIP-1β: 0.38 - 6,000 pg/mL
  • IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-18, MDC: 0.64 - 10,000 pg/mL
  • FLT-3L: 0.96 - 15,000 pg/mL
  • GROα, IFNγ, IL-3, IL-17A, RANTES, TGFα: 1.3 - 20,000 pg/mL
  • IL-1β, IL-1RA, TNFβ: 1.6 - 25,000 pg/mL
  • GM-CSF, IL-10, IP-10, VEGF-A: 2.6 - 40,000 pg/mL
  • EGF, Eotaxin, IL-12 (p70), IL-15, MCP-1, MIP-1α: 3 - 50,000 pg/mL
  • G-CSF, IL-1α: 4.8 - 75,000 pg/mL
  • IL-12 (p40), IL-13, MIG, TNFα: 6.4 - 100,000 pg/mL
  • IFNα2, MCP-3: 8 - 125,000 pg/mL
  • PDGF-AB/BB: 9.6 - 150,000 pg/mL
  • sCD40L, IL-22, PDGF-AA: 13 - 200,000 pg/mL
  • IL-27: 16 - 250,000 pg/mL
  • FGF-2: 26 - 400,000 pg/mL
  • Fractalkine, IL-17F: 32 - 500,000 pg/mL
  • IL-17E/IL-25, M-CSF: 40 - 625,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information
Specifications